摘要
Abstract
Objective To observe the morphology and characteristics of hippocampus neurons in adult rats in vitro.Method The hippocampus tissues from adult rats were collected, then digested into cell suspension by using 0.125％ trypsin.Cell suspension was planted into wells and observed at day 1, 3, 7, 10, 13, and 15 after incubation.Neurons specific Enolase (NSE) antibody, recognized specifically NSE antigen in cultured cells, was used to identify neurons by SP-two-step staining method.Results During 1-3 days, the extensive bodies of floating cells and tissue blocks were seen, but a few cells attached to the bottom of well.The growth of attached cells was also extensively slow, specifically within 1 week, while it began to grow quickly after 7 days,and maintained till 15 days.Amazingly, cells exhibited aging character with more particles in cytoplasm after 15 days.NSE staining showed that cultured cells were positive staining by neuronal specific enalose antibody.Conclusion Adult hippocampus neurons grow slowly during the first week, but they are better from 7 day to 15,then begin to degenerate after 15 days in vitro.This suggests that neurons from hippocampus of adult rats cultured in 10 days could be better for related neurobiological studies.关键词
大鼠/神经细胞/海马/细胞培养/免疫组化Key words
Adult rats/ Hippocampus neurons/ NSE staining/ Culture分类
生物科学
