昆明医学院学报2011，Vol.32Issue(6)：49-52,4.
汽车安全气囊上微量检材DNA检测方法的探讨
Trace DNA Detection Methods in Airbag of Vehicle
摘要
Abstract
Objective To disc uss the effective DNA detection method for the trace of human epithelial cells exfoliated on airbag of vehicle. Methods The trace DNA of human epithelial cells extracted form the airbag was detected by the methods of cotton carrier direct amplification, small volume of Chelex-100, and the detection of magnetic beads, and the results were analyzed and compared in order to obtain a valid method. Rosults Cotton carrier direct amplification method and the detection of magnetic beads method can get the satisfactory STR loci genotyping results of the shedding epithelial cell nuclear DNA of human, whereas the small volume of Chelex-100 method can not obtain the results of STR loci genotyping. Conclusions Cotton carrier direct amplification method and detection of magnetic beads method can detect the trace of human DNA effectively, obtain the results of STR leci genotyping, and improve the DNA detection rate. Especially cotton carrier direct amplification method, which is simple, saving time and reagent costs, and has a high application value in the actual detective work of biological evidence.关键词
汽车安全气囊/微量人体DNA检测方法/STR基因座/个人识别/法医学应用Key words
Airbag/ Trace DNA detection of human/ STR loci/ Personal identification/ Forensic medicine application分类
社会科学引用本文复制引用
董雪梅,郭刚,陈立方,王小梅,许冰莹..汽车安全气囊上微量检材DNA检测方法的探讨[J].昆明医学院学报,2011,32(6):49-52,4.基金项目
云南省应用基础研究基金资助项目(2007CO74M) （2007CO74M）
云南省科技厅-昆明医学院联合专项基金资助项目(2010CD226) （2010CD226）
