昆虫学报2011，Vol.54Issue(7)：828-837,10.
基于线粒体CO Ⅱ基因序列的双斑长跗萤叶甲中国北方地理种群的遗传多样性研究
Genetic diversity of geographic populations of Monolepta hieroglyphica( Motschulsky ) ( Coleoptera: Chrysomelidae ) from North China estimated by mitochondrial CO Ⅱ gene sequences
摘要
Abstract
Monolepta hieroglyphica (Motschulsky) (Coleoptera: Chrysomelidae ) has become more serious on maize in some maize planting areas of North China since 2001. To investigate the level of genetic diversity, genetic differentiation and gene flow among different populations and within populations of M. hieroglyphica in North China, partial sequences (484 bp) of mtDNA CO Ⅱ (mitochondrial cytochrome oxidase subunit Ⅱ ) gene in 26 geographic populations from North China were sequenced and analyzed. In the total 515 individuals, 28 variable sites and 15 haplotypes were observed. Phylogenetic tree of 15 CO Ⅱ haplotypes exhibited two main clusters. The mean haplotype diversity of all populations was 0.257, varied from 0. 100 to 0.515 within populations. The overall Fst value was 0.585, Gst was 0.417, and the average gene flow (Nm) among the 26 populations was 0.35. Molecular variance (AMOVA) analysis indicated that a high proportion of the total genetic variance was attributable to variations among populations (58.58％). The Tajima' s D neutrality test results suggest that for M.hieroglyphica in North China there might be no population expansion. These results indicate that the level of gene flow among different populations is very low, and there is obvious differentiation among M. hieroglyphica populations in North China.关键词
双斑长跗萤叶甲/线粒体COⅡ/地理种群/遗传多样性/遗传分化Key words
Monolepta hieroglyphica/ mtDNA CO Ⅱ/ geographic population/ genetic diversity/genetic differentiation分类
数理科学引用本文复制引用
梁日霞,王振营,何康来,丛斌,李菁..基于线粒体CO Ⅱ基因序列的双斑长跗萤叶甲中国北方地理种群的遗传多样性研究[J].昆虫学报,2011,54(7):828-837,10.基金项目
现代农业产业技术体系建设专项(CARS-02) （CARS-02）
公益性行业(农业)科研专项(200903004) （农业）
