农业生物技术学报2011，Vol.19Issue(3)：407-416,10.DOI:10.3969/j.issn.1674-7968.2011.03.002
番茄茸毛相关基因ShMYB1的克隆与表达分析
Cloning and Expression Analysis of ShMYB1 Related to the Trichome Initiation in Tomato (Solanum habrochaites)
摘要
Abstract
The trichome has diverse biological functions in tomato, in order to explore the gene functions involved in trichome formation, a novel gene (named as ShMYB1) related trichome initiation was isolated from LA1777, using RACE (rapid amplification of cDNA ends) technology. The full-length sequence of ShMYB1 cDNA was 1 350 bp and encoded a protein of 338 amino acids. The ShMYB1 had a conserved R2R3 MYB domain and a specific subgroup 9 motif. The expression profiles of ShMYB1 were analyzed with Real-time PCR in different tissues and developmental stages, the results showed that the gene was expressed at a high level in leaves and flowers, but no expression was detected in roots, stems or fruits, and no significant differences at different stages of leaf development, but the highest expression occurred in young buds, gradually declined during bud development. Also significant differences of ShMYB1 expression were detected between several trichome mutants and their wild types. These results suggested that the ShMYB1 gene may be involved in the initiation of trichome and the development of flowers.关键词
番茄(Solanum/habrochaites)/MYB转录因子/基因克隆与表达/茸毛Key words
Tomato (Solanum habrochaites)/ MYB transcription factor/ Gene cloning and expression/ Trichome引用本文复制引用
王涛涛,Maarten A.Jongsma,余楚英,李汉霞,张俊红,杨长宪,叶志彪..番茄茸毛相关基因ShMYB1的克隆与表达分析[J].农业生物技术学报,2011,19(3):407-416,10.基金项目
本研究由教育部新教师基金(No.200805041045)、国家自然科学基金(No.31000912)和中国一荷兰政府合作项目(No.AF01CH05)共同资助 （No.200805041045）
