农业生物技术学报2011，Vol.19Issue(3)：455-461,7.DOI:10.3969/j.issn.1674-7968.2011.03.008
农杆菌介导的CFL(Cucumber-FLO-LFY)基因遗传转化大岩桐
Transformation of CFL (Cucumber-FLO-LFY) Gene in Gloxinia(Sinningia speciosa) Mediated by A grobacterium tumefaciens
摘要
Abstract
Different Agrobacterium-mediated protocols were used to evaluate the transformation efficiency of gloxinia (Sinningia speciosa), and CFL (Cucumber-FLO-LFY) gene was introduced into gloxinia to investigate its potential to promote early flowering.The plant expression vector pCA-CFL was constructed by inserting CFL gene into the plant high-efficient expression vector pCAMBIA13011.To restrain the growth of untransformed cells, we confirm the hygromycin (Hyg) filtration pressure (20mg/L) through explants culture treated with different Hyg concentrations.CFL gene was transformed into leaves of gloxinia mediated by Agrobacterium tumefaciens and GUS (β-glucuronidase) test showed that the protocol of ultrasonic 10 s treatment was the most efficient.After two selection cultures, dozens of Hyg-resistant regenerated green shoots were obtained.PCR and Southern dot blot analysis revealed that CFL gene had been integrated into the gloxinia genome.Transgenic seedlings were grown under long-day condition until maturity.The analysis showed that most of transgenic gloxinia plants (71％)flowered 26～32 days earlier than wild-type plants, and had terminal flowers emerging directly from shoot apex with no inflorescence branches.Our results imply that CFL act as a functional homolog of LEAFY (LFY) in g1oxinia.关键词
CFL(Cucumber-FLO-LFY)基因/转基因大岩桐/农杆菌介导Key words
CFL(Cucumber-FLO-LFY)gene/ Transgenic gloxinia/ Agrebacterium-mediated引用本文复制引用
张明哲,王利琳,叶丹,吴志毅,陈曦,张晓峰,边红武,韩凝,朱睦元..农杆菌介导的CFL(Cucumber-FLO-LFY)基因遗传转化大岩桐[J].农业生物技术学报,2011,19(3):455-461,7.基金项目
本研究由国家质检总局科技计划项目(No.2011IK249)、浙江省科技厅面上项目(No.2009C32076)和浙江出入境检验检疫局科研项(No.ZK200958) （No.2011IK249）
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