农业生物技术学报2011，Vol.19Issue(3)：462-469,8.DOI:10.3969/j.issn.1674-7968.2011.03.009
植物同源结构域(PHD)-finger家族转录因子OsPHD1的过量表达可提高水稻的耐逆性能
Overexpression of a Plant Homedomain (PHD)-finger Transcription Factor,OsPHD1, Can Enhance Stress Tolerance in Rice
摘要
Abstract
OsPHD1 is a member of the plant homedomain (PHD)-finger transcription factor family in rice(Oryza sative). Expression of OsPHD1 is induced under various stresses, including drought, high salinity and cold treatments. By using Agrobacterium-mediated method, OsPHD1 was successfully introduced into rice cultivar Zhonghua 11 (Oryza s ativa ssp. japonica) and T1 generation of transgenic rice was achieved. The results indicated that overexpression of OsPHD1 in rice could significantly improve more than 43.3％, 60 ％ and 25％ respectively in drought (70％ ～95％ relative water content), high saalinity (200 mmol/L) and cold tolerance (4～8℃) of transgenic plants, and this phenotype was well confirmed in transgenic plants of T2 generation, meanwhile, transgenic plant was almost not different to control in some field characters. Subcellular localization assay of OsPHD1 by using transient expression of OsPHD1 ::GFP fusion protein in onion (A llium cepa) cells revealed nuclear localization of OsPHD1 protein. Results of qRT-PCR suggested that OsPHD 1 protein regulated the anti-reversibility of plants by regulating the stress response of gene expression. Results imaged that OsPHD1 gene has an important application prospect for the anti-reversibility ofpalnt breeding.关键词
OsPHDl/转基因/非生物胁迫/亚细胞定位Key words
OsPHD1/ Transgenic rice/ Abiotic stress/ Subcellular localization引用本文复制引用
刘雨,刘春霞,李早霞,夏勉,江海洋,程备久,周君莉,朱苏文..植物同源结构域(PHD)-finger家族转录因子OsPHD1的过量表达可提高水稻的耐逆性能[J].农业生物技术学报,2011,19(3):462-469,8.基金项目
本研究由国家科技支撑计划(No.2009BADA6B001)和国家自然科学基金(No.11075001)共同资助 （No.2009BADA6B001）
