农业生物技术学报2011，Vol.19Issue(3)：501-506,6.DOI:10.3969/j.issn.1674-7968.2011.03.015
抵抗素对猪前脂肪细胞增殖与分化的影响
Effects of Resistin on Proliferation and Differentiation of Porcine Preadipocyte In vitro
摘要
Abstract
Resistin play important roles in organism lipometabolism and energy metabolism.The present work was used to study the effects of resistin on proliferation and differentiation of porcine preadipocyte. In this paper, the preadipocytes were isolated from subcutaneus adipose tissue of 1-day-old crossbred piglet, and the preadipocytes were respectively cultivated in the DMEM/F12 culture media containing 0, 50, 100,150 and 200 μg/L recombinant resistin. The changes of the cell morphology were observed, and the tfiglyceride content and the activity of lipoprotein lipase of the cells were determined to evaluate proliferation and differentiation of porcine preadipocyte. Results showed that the proliferation of porcine preadipocytes were significantly promoted by 0 to 100 μg/L resistin supplementation in the media, the activity of lipoprotein lipase of the cells was also significantly increased. As the resistin concentration in the culture media came to 100～200 μg/L,the facilitory effect of resistin on proliferation of porcine preadipocyte was declined. Moreover, as resistin concentration increased, the differentiation of porcine preadipocyte was significantly strengthened, the intracellular triglyceride synthesize and fatty droplet aggregation were promoted simultaneously. The results indicated that the resistin has dual effects on growth of porcine preadipocyte.关键词
抵抗素/前脂肪细胞/增殖/分化Key words
Resistin/ Preadipocyte/ Proliferation/ Differentiation引用本文复制引用
陈育峰,刘铀,陈绍红,刘艳芬..抵抗素对猪前脂肪细胞增殖与分化的影响[J].农业生物技术学报,2011,19(3):501-506,6.基金项目
本研究由广东海洋大学人才引进项目(N0.0612053)资助 （N0.0612053）
