农业生物技术学报2011，Vol.19Issue(3)：507-512,6.DOI:10.3969/j.issn.1674-7968.2011.03.016
脂联素(A dipoQ)及其受体基因(A dipoR1and A dipoR2)在猪背最长肌和腰大肌中的表达差异
Expression Changes of Adiponection (A dipoQ) and Its Receptor Genes (A dipoR1 and A dipoR2) in Porcine Longissimus Dorsi and Psoas Major Muscles
摘要
Abstract
Adiponection (AdipoQ), a protein hormone secreted from adipose tissue, modulates a number of metabolic processes, including fatty acid catabolism and regulation of insulin sensitivity. The expression changes of AdipoQ and its receptor genes (AdipoR1 and AdipoR2) in longissimus dorsi and psoas major muscles of Rongchang (a fatty, indigenous, Chinese breed) and Landrace pigs (a leaner, Western breed) were measured using a Real-time fluorescence quantitative PCR approach. Results showed that the A dipoQ, A dipoR 1 and A dipoR2 were ubiquitously expressed in all tissues analyzed, nonetheless, their mRNA abundances existed notable differences among pig breeds, genders and tissue types. The mRNA abundances of AdipoQ and AdipoR1 genes exhibited a negative correlation with the POI (porcine obesity index) and IMF (intramuscular fat) content traits, and a positive correlation with longissimus dorsi muscle area. Additionally, the mRNA abundance of AdipoR2 gene exhibited a strongly negative correlation (P＜0.05) with IMF content. These results are in accordance with the well-annotated function of AdipoQ and its receptor genes, which increase fatty acid oxidation and decrease fat deposition, and highlight A dipoQ, A dipoR1 and A dipoR2 as candidate genes for porcine meat quality and carcass traits.关键词
猪/脂联素/脂联素受体/肌肉生长/脂肪沉积Key words
Pig/ Adiponectin(AdipoQ)/ Adiponectin receptors/ Muscle growth/ Fat deposition引用本文复制引用
罗宗刚,李祥,李明洲,李学伟..脂联素(A dipoQ)及其受体基因(A dipoR1and A dipoR2)在猪背最长肌和腰大肌中的表达差异[J].农业生物技术学报,2011,19(3):507-512,6.基金项目
本研究由转基因生物新品种培育重大专项(No.2009ZX08009-155B和No.2008ZX08006-003)、国家自然科学基金(No.30901024)和国家公益性行业(农业)科研专项(No.NYHYZX07-034)共同资助 （No.2009ZX08009-155B和No.2008ZX08006-003）
