农业生物技术学报2011，Vol.19Issue(3)：530-534,5.DOI:10.3969/j.issn.1674-7968.2011.03.019
绵羊口蹄疫病毒整联蛋白受体αv亚基基因不同组织表达差异
Relative Quantification of mRNA Transcription of αv Subunits Relevant to Food and mouth disease virus (FMDV) Receptors in Different Tissues of Sheep by Real-time Quantitative RT-PCR
摘要
Abstract
For establishing an assay of Real-time PCR to detect distribution and expression level of sheep Food and mouth disease virus (MDV) integrin receptor αv submit in different tissue, in this study, a pair of primers was designed according to the published nudeotide sequence of integrin receptor αv submit.And tissue expression profile of sheep (Ovisaries) integrin αv in different tissue was detected by Real-time PCR.The results showed that the integrin receptor αv submit was generally expressed in 19 kinds of tissues with different level.The highest expression level was showed in breast, the next was in hoof, and the lowest was in muscle tissue.We have established the Real-time PCR method which can be used to confirm tissue profile of sheep FMDV integrin receptor αv submit.This study provides basic data for further study tissue tropism of FMDV and molecular detection method.关键词
绵羊/口蹄疫病毒(FMDV)受体αv/mRNA转录水平/荧光定量PCRKey words
Sheep/ Food and mouth disease virus (FMDV) integrin receptor αv/ mRNA transcription level/Real-time quantitative RT-PCR引用本文复制引用
吾热力哈孜,陈创夫,徐宁迎,胡圣伟,王遵宝,马柿委..绵羊口蹄疫病毒整联蛋白受体αv亚基基因不同组织表达差异[J].农业生物技术学报,2011,19(3):530-534,5.基金项目
本研究由国家转基因生物新品种培育重大专项(No.2009ZX08005-003B)资助 （No.2009ZX08005-003B）
