农业生物技术学报2011，Vol.19Issue(3)：549-556,8.DOI:10.3969/j.issn.1674-7968.2011.03.022
四环素降解菌的选育、鉴定及其降解特性
Screening, Identification and Degradation Characteristics of Tetracycline-degrading Strains
摘要
Abstract
Tetracycline waste residue is considered to be a good protein source for animal feed, since it contains a high proportion of protein and a relatively nutritional balance in amino acids composition. On the other hand,tetracycline waste residue also has a high level of tetracycline, which will limit its usage in animal feed industry. It may be feasible to eliminate tetracycline in the waste via microorganism fermentation method. Thus, the present study was conducted to obtain tetracycline-degrading strains. Two tetracycline-degrading strains, named TD2 and TD3, were isolated fiom soil contaminated by tetracycline. Based on the results of phenotype features,physio-biochemical identification and the phylogenetical analyses of 16S rDNA sequence, TD2 and TD3 were identified to Brevundimonas diminuta and Ochrobactrum anthropi, respectively. Both TD2 and TD3 could grow in the medium containing tetracycline as only carbon source. The highest efficacy of TD2 was achieved when it was incubated in the medium consisted of 0.5％ peptone and 0.015％ CuSO4, whereas that of TD3 was obtained in the medium composed of 0.5％ glucose, 1.5％ beef extract, 0.015％ Cu SO4. Identically, both TD2 and TD3 had an optimal inoculation ratio of 1％, and tetracycline degradation rate proportional to ventilation, as well as an optimal culture condition of 30℃ for five days. After the optimal incubation, the tetracycline degradation ratio for the two strains could be up to 90％. Our results indicated that TD2 and TD3 were the microorgainism candidates for the tetracycline waste residue fermentation process by which tetracycline can be eliminated.关键词
四环素降解菌/筛选/菌株鉴定/降解性能Key words
Tetracycline-degrading strain/ Strain screening/ Strain identification/ Degradation characteristics引用本文复制引用
许晓玲,李卫芬,雷剑,王敏奇..四环素降解菌的选育、鉴定及其降解特性[J].农业生物技术学报,2011,19(3):549-556,8.基金项目
本研究由教育部新世纪优秀人才支持计划项(No.NCET-06-0913)、浙江省自然科学基金(No.Y2100925)和温州科技计划项目(No.Y20100274)共同资助 （No.NCET-06-0913）
