农业生物技术学报2011，Vol.19Issue(3)：577-582,6.DOI:10.3969/j.issn.1674-7968.2011.03.026
转基因玉米LY038转化事件的特异性检测
Event-specific Transgenic Detection of Genetically Modified Maize LY038
摘要
Abstract
Genetically modified maize with high lysine LY038 has been widely applied in animal feed, but so far there has been no report about the method of amplification for flanking sequence of LY038 and its event-specific qualitative PCR detection. In this study, the 5'- flanking sequence of integrated gene-construct of genetically modified maize LY038 was characterized by modified adapter-linked PCR (M-AL-PCR). According to the 5'flanking sequence, the event-specific primers were designed, and the event-specific qualitative detection of genetically modified plant LY038 was established to produce a 175 bp product. With genetically modified maize (Zea mays L.) LY038, MIR604, Bt176, Btll, MON810, MON863, GA21, NK603, non-genetically modified maize, genetically modified rice (Oryza sativa L.) CrylC* and Cry2A*, genetically modified soybean (Glycine max) Roundup Ready and genetically modified oilseed rape (Brassica campestris L.) GT73 as materials, this method developed in this work proved to be highly specific and sensitive, and the limit of detection was 0.1％. It is suggested that the developed qualitative detection system can accurately, fast and efficiently identify the genetically modified maize LY038 and its derivates.关键词
转基因玉米/修饰接头连接PCR/LY038/转化事件特异性检测/侧翼序列Key words
Genetically modified maize/ Modified adapter-linked PCR (M-AL-PCR)/ LY038/ Event-specific transgenic detection/ Flanking sequence引用本文复制引用
翟志芳,许文涛,张南,闫兴华,王一南,罗云波,黄昆仑..转基因玉米LY038转化事件的特异性检测[J].农业生物技术学报,2011,19(3):577-582,6.基金项目
本研究由国家自然科学基金(No.30800770)和国家转基因生物重大专项(No.2008ZX08012-001)共同资助 （No.30800770）
