青岛大学医学院学报2011，Vol.47Issue(4)：293-295,3.
肌醇六磷酸对肝癌HepG2细胞增殖的影响
EFFECT OF INOSITOL HEXAPHOSPHATE ON MULTIPLICATION OF LIVER CANCER CELL HepG2
摘要
Abstract
Objective To study the effect and mechanisms of inositol hexaphosphate (IP6) on proliferation of human liver cancer cell HepG2. Methods HepG2 cells were cultured in vitro and treated with various concentrations of IP6. MTT assay was used to observe the effect of IP6 on proliferation of HepG2 cells, and plate colony-forming assay to detect the colony-forming efficiency. The expressions of P53 and P21 were tested by immunocytochemical technique. Results Compared with the control, IP6 group (1.0, 2.0, 3.0 mmol/L) declined the colony formation of the cells and raised the clone-inhibiting rate. IP6 inhibited the expression of P53, and promoted the expression of P21. Conclusion IP6 plays a role in inhibiting the proliferation and declining the clonality of HepG2 cells. The mechanism of inhibiting the proliferation of HepG2 cells is, probably, by regulating the expressions of P53 and P21, resulting in blockage of the cell cycle.关键词
植酸/HepG2细胞/细胞增殖Key words
phytic acid/ HepG2 cells/ cell proliferation分类
医药卫生引用本文复制引用
杨伟品,宋扬,孟显锋..肌醇六磷酸对肝癌HepG2细胞增殖的影响[J].青岛大学医学院学报,2011,47(4):293-295,3.基金项目
山东省博士基金资助项目(2007BS03042) （2007BS03042）
