青岛大学医学院学报2011，Vol.47Issue(4)：320-323,4.
腹部辐射诱发大鼠肠黏膜损伤模型的建立
CREATION OF MODEL OF INTESTINAL MUCOSA STRUCTURE DAMAGE INDUCED BY ABDOMINAL RADIATION IN RATS
摘要
Abstract
Objective To establish a stable animal model that consistent with the requirements for research of radiation damage of intestinal mucosal barrier. Methods SD rats were randomly divided into control group (group A, 10 rats) and radiation groups B, C, D (20 in each group). The rats in groups B, C, and D were given single fraction irradiation on abdomen with linear accelerator, the dosage being 8. 5, 9.0, and 9.5 Gy, respectively, the radiation scope was from the xyphoid to anus. No radiation was given to the rats in group A. At day 4 after radiation, a segment of intestine, 10 cm from Treitz's ligament, was taken for making specimen. The length of crypt-villus axis (CVA) was measured light-microscopically, and intestinal mucous membrane structure observed electron-microscopically. Plasma endotoxin levels were detected by using tachypleus amebocyte lysate. Results At day 4 after radiation, death rates of the rats in groups B, C, and D were 10％, 30％, and 60％, respectively. A comparison of the length of CVA among the four groups showed: the difference between groups A and B was not significant (P＞0.05) ; the length of CVA was shorter in group C than that in groups A and B; and that in group D was shorter than that in groups A, B, and C, the differences were all significant (F= 100.48,q=4.617-18.560,P＜0.05). Plasma endotoxin concentration of group C was higher that that of groups A and B, that of group D was higher than that of groups A, B and C (F=50.468,q=5.380-15.898,P＜0.05). Electron microscopically, intestinal epithelial cell shedding, ulcer formation, no normal cellular structure, exposure of basal lamina, and bacteria penetrating the stratum mucosum could be seen. Conclusion A model of radiation-induced damage of intestinal mucosal structure in the rat is created successfully. The model of radiation enterocolitis caused by total abdominal irradiation of a dose of 9. 0 Gy is applicable to research for radiation damage of mucosa barrier of intestine.关键词
肠黏膜/辐射损伤,实验性/腹部/模型,动物Key words
intestinal mucosa/ radiation injuries/ experimental/ abdomen/ model, animal分类
医药卫生引用本文复制引用
韩磊,单信芝,刘芝军,王娟娟..腹部辐射诱发大鼠肠黏膜损伤模型的建立[J].青岛大学医学院学报,2011,47(4):320-323,4.基金项目
山东省卫生厅课题(HZ023) （HZ023）
