摘要
Abstract
Objective To study the effects of serum containing optimized gout mixtures (OGM)-α Chinese medicinal formulae-on expressions of TNF-α and IL-1β in human umbilical vein endothelial cells (HUVECs) stimulated by urate. Me thods Thirty-six Wistar rats were randomly divided into gout mixture (GM) group, OGM group and control group. The rats in each group were given tenfold concentration of GM, OGM, and normal saline, respectively, twice a day, for one week, intragastrically. The rats were sacrificed one hour after the last medication. The serum was diluted to 80％, 40％ and 10％ concentrations by culture solution. HUVECs were cultured as routine, and divided into control group, model group, GM group, and OGM group when the fusion reached 80％. In each group, it was redivided into three subgroups as high-, moderate- and low-concentration.The normal control group was stimulated with 100 mg/L culture solution, the remaining groups were stimulated with 100 mg/L urate solution. The 80％, 40％, and 10％ concentrations of GM and OGM were used to intervene GM group and OGM group; the model group and normal control group were intervened by serum of normal rat with the same concentration as above. After 48 hours, the s upernate fluid was collected and centrifugated, and the expressios of TNF-α and IL-1β determined by enzyme-linked immunosorbent assay. Results The levels of TNF-α and IL-1β in each model group of various concentrations were higher than that in corresponding normal groups (F= 179.95- 1 122.88, q = 2.42 - 67. 90, P＜0.05) ; the levels of IL-1β in high- and moderateconcentration OGM were significantly lower than that in model groups of corresponding concentration (q= 24. 03,22. 99; P＜ 0. 05). Conclusion The inhibition action of OGM on IL-1β is one of the mechanisms to ease the inflammatory lesion of human vascular endothelial cells induced by urate.关键词
痛风合剂优化方/脐静脉/内皮细胞/尿酸/肿瘤坏死因子α/白细胞介素1βKey words
optimized gout mixture/ umbilical vein/ endothelial cells/ uric acid/ tumor necrosis factor-alpha/ interleukin1 beta分类
医药卫生
