天然产物研究与开发2011，Vol.23Issue(3)：494-497,4.
HPLC法测定银州柴胡中五种皂苷的含量
Determination of five saikosaponins in Bupleurum yinchowense by HPLC
摘要
Abstract
A method was established for determining five saikosaponins simultaneously, including saikosaponins a, c, d, e and f in the root of Bupleurum yinchowense by HPLC for the first time. Separation was achieved by A Hibar ? RT C18 column (250 mm ×4.6 mm,5 μm) using acetonitrile and water as the mobile phase with gradient elution at the flow rate of 1.0 mL/min,column temperature at 30 ℃ and the wavelength of UV detection was 210 nm. There were good linear correlations between the studied concentrations of the five saikosaponins and their chromatographic peak areas, and the correlation coefficients were 0.9992-0.9998. The recoveries of saikosaponins a and d were 101.5 and 98.5％ ,respectively. The RSD of precision and accuracy was less than 3％. This method is simple,accurate and reproducible,and also provides a reference for establishing the multiple-value quality control method for Radix Buplerui and the use of B.yinchowense as a medicine.关键词
HPLC/银州柴胡/柴胡皂苷a/柴胡皂苷c/柴胡皂苷d/柴胡皂苷e/柴胡皂苷fKey words
HPLC/Bupleurum yinchowense/ saikosaponin a/ saikosaponin c/ saikosaponin d/ saikosaponin e/ saikosaponinf分类
医药卫生引用本文复制引用
符颖,朱占军,黄帅,斯建勇,魏建和,潘瑞乐..HPLC法测定银州柴胡中五种皂苷的含量[J].天然产物研究与开发,2011,23(3):494-497,4.基金项目
创新药物研究开发技术平台建设(2009ZX09301-003) （2009ZX09301-003）
