四川医学2011，Vol.32Issue(7)：978-981,4.
微囊化异种嗅球组织移植联合L-NAME治疗脊髓损伤的实验研究
Experimental study on exnotransplantation of microencapsulated olfactory bulb combined with L-NAME for the treatment of spinal cord injury
摘要
Abstract
Objective To explore the protective effects and mechanism of exnotransplantation of microencapsulated olfactory bulb combined with L-NAME in rats at the early stages of spinal cord injury. Methods Healthy SD rats were divided into three groups as following: injury control group, microencapsulated transplantation of rabbit olfactory bulb cells group, microencapsulated transplantation of rabbit olfactory bulb cells combined with L-NAME group, the later two groups were quantified at 1, 3,7, 14, and 28 d post spinal cord injury. Spinal Evans blue content was examined with the ultraviolet spectrophotometer; Spinal cord water content was detected by caculating dry/wet weight. The change of MMP-9 expression after spinal cord injury was determined by immunohistochemical method. BBB score was designed to assess its behavioral ability. Results Evans blue content and expression of MMP-9 were significantly were significantly increased, vasogenic spinal cord edema, and loss of motor function in rats after spinal cord irjury. Permeability of blood-spinal barrier and vasogenic spinal cord edema, MMP-9 were decreased, motor function was recoveried in rats after they were treated with microencapsulated transplantation of rabbit olfactory bulb cells combined with L-NAME. Conclusion Exnotransplantation of microencapsulated strategy of olfactory ensheating cells was effective in the repair of spinal cord injury in some extent. There may be a synergistic effect between olfactory ensheating cells and L-NAME.关键词
脊髓损伤/异种移植/嗅鞘细胞/L-硝基-精氨酸甲酯/基质金属蛋白酶-9Key words
spinal cord injury/ exnotransplantation/ olfactory ensheating cells/ NG-nitro-L-arginine methyl ester/ matrix metalloproteinase-9分类
医药卫生引用本文复制引用
王航辉,白延斌,李小记,王璐,朱文侠,韩振奎,赵琳..微囊化异种嗅球组织移植联合L-NAME治疗脊髓损伤的实验研究[J].四川医学,2011,32(7):978-981,4.基金项目
陕西省卫生厅科学研究基金项目资助(编号:2010H25) （编号:2010H25）
陕西省自然科学基金资助项目(编号:08JK502) （编号:08JK502）
延安市科技计划项目资助(编号:2009KS-22) （编号:2009KS-22）
延安大学专项科研基金资助项目(编号:YD2008-39) （编号:YD2008-39）
