畜牧兽医学报2011，Vol.42Issue(6)：845-850,6.
用抗虫体表膜抗原抗体测定犬细粒棘球绦虫粪抗原
Using Antibodies against Tegument Antigens for Copro-ELISA to Detect Echinococcus granulosus in Dogs
摘要
Abstract
The study was aimed at determining whether antibodies against worm tegument antigens can be used for copro-ELISA to detect Echinococcus granulosus in dogs. In order to produce specific antibodies used for sandwich (copro)-ELISA, we isolated antigens from E. granulosus adult worm tegument. The proteins were used to immunize mice, rabbits and sheep to generate antibodies. ELISA and Western blot analysis were used to identify antigenicity and quality of the serum antibodies. Immunohistochemistry was used to indentify the localization of the proteins in the parasite. Copro-ELISA was used to determine the specificity of the immunodiagnosis for detecting E. granulosus. infection in dogs. Scrum titer analysis showed that E. granulosus tegument antigens were antigenic with serum titers reaching to 1 : (80000-320000) by ELISA. The antibodies recognized antigens from different stages of the parasite. Immunolocalization analysis revealed that the antigens located in the tegument of adult E. granulosus. The antibodies specifically recognized the antigens from E. granulosus with no reaction to the proteins isolated from Multiceps multicepes and T. hydatigena. The results suggest that E. granulosus tegument antigens are a source of antigens for generating antibodies for copro-ELISA to detect the worm antigens in dog feces.关键词
细粒棘球绦虫成虫表膜抗原抗体/双抗体夹心ELISA粪抗原Key words
Echinococcus granulosus adult worm tegument Ag/ copro-ELISA/ coproantigen分类
农业科技引用本文复制引用
古努尔·吐尔逊,金映红,张文宝,米晓云,张壮志,石保新,吐尔洪·依米提,张旭,巫剑,赵莉,阿曼古丽·马木提..用抗虫体表膜抗原抗体测定犬细粒棘球绦虫粪抗原[J].畜牧兽医学报,2011,42(6):845-850,6.基金项目
新疆畜牧科学院青年科研基金项目(2008QJ03) （2008QJ03）
公益性行业(农业)科研专题项目(200903036-12) （农业）
