畜牧兽医学报2011，Vol.42Issue(6)：880-884,5.
中国家驴生长激素基因内含子2的遗传多样性分析
Genetic Polymorphisms of the Intron 2 of GH Gene in Chinese Domestic Donkeys
摘要
Abstract
To reveal the genetics diversity of different donkey breeds at molecular level and provide some bases for the breed resource evaluation and utilization of donkeys in China,seven domestic donkey breeds ( Linxian, Guanzhong, Xinjiang, Guangling, Huaibei, Dezhou, Jinnan) were used as experimental populations,the genetic variations of GH gene was researched by PCR-SSCP method. The results were as follows: The polymorphic PCR-SSCP sites were in the intron 2 of GH gene. Three haplotypes with the percentage of 1. 7％ in 174 animals were obtained. The haplotype diversity was high as 0. 678 and 0. 542 for the Linxian and Huaibei donkeys,and low as 0. 077 for Xinjiang donkey,with that of 0. 409 and 0. 462 for Guangling and Guanzhong donkeys,respectively, with that of 0. 355 and 0. 304 for Dezhou and Jinnan donkeys,respectively. The amplified fragments of A and B haplotypes were cloned and sequenced. The result showed that the fragment of B haplotype had one substitution mutation at 735 site(G→C) ,the fragment of A haplotype had one substitution mutation at 869 site(G→T). The results confirm that there are polymorphisms in intron 2(214 bp) of GH gene for the first time.关键词
GH基因/PCR-SSCP/遗传多态性Key words
GH gene/ PCR-SSCP/ genetics diversity分类
农业科技引用本文复制引用
关学敏,朱文进,苏咏梅..中国家驴生长激素基因内含子2的遗传多样性分析[J].畜牧兽医学报,2011,42(6):880-884,5.基金项目
河北科技师范学院博士启动基金(2006D006) （2006D006）
青年基金和河北省自然科学基金(C2007000739) （C2007000739）
河北省教育厅科学研究项目(22010137) （22010137）
秦皇岛科学研究与发展项目(201001A155) （201001A155）
