畜牧兽医学报2011，Vol.42Issue(6)：892-898,7.
PRRSV GP5蛋白和CSFV E2蛋白在杆状病毒囊膜表面的共展示及展示蛋白的免疫原性分析
Baculovirus Surface Display of GP5 Envelope Glycoprotein of PRRSV and E2 Envelope Glycoprotein of CSFV and Immunogenicity Analysis of the Displayed Proteins
摘要
Abstract
The aim of this study was to develop porcine reproductive and respiratory syndrome (PRRS) and classical swine fever (CSF) divalent genetic engineering subunit vaccine based on baculovirus surface display system. One recombinant baculoviruses BacSC-Dual-GP5-E2 expressing histidine-tagged PRRSV GP5 protein and CSFV E2 protein with the CTD and TM derived from baculovirus envelope protein gp64 were constructed. The recombinant baculoviruses were analyzed by Western blot,immunofluorescence confocal microscopy,immunogold electron microscopy,animal vaccine tests and lymphocyte proliferative responses. After infection, GP5 and E2 protein was expressed and anchored on the plasma membrane of Sf-9 cells,as revealed by confocal microscopy. Immunogold electron microscopy demonstrated that the BacSC-Dual-ORF5-E2 was displayed GP5 and E2 proteins on the viral surface. Animal vaccine tests showed that BacSC-Dual-ORF5-E2 elicited significantly higher anti-PRRSV and anti-CSFV antibody titers in the treated mouse model. Lymphocyte proliferative responses results indicated that recombinant BacSC-DualORF5-E2 also induced an obvious cellular immune response. The recombinant baculoviruses BacSC-Dual-ORF5-E2 that surface display GP5 and E2 protein were successfully constructed. The present results demonstrate that the recombinant baculoviruses can be a potential bivalent genetic engineering vaccine against PRRSV and CSFV mixed infections.关键词
杆状病毒表面展示系统/PRRSV/CSFV/GP5蛋白/E2蛋白Key words
baculovirus surface display/ PRRSV/ CSFV/ GP5 protein/ E2 protein分类
农业科技引用本文复制引用
许信刚,王志昇 ,李兆才,张琪,童德文..PRRSV GP5蛋白和CSFV E2蛋白在杆状病毒囊膜表面的共展示及展示蛋白的免疫原性分析[J].畜牧兽医学报,2011,42(6):892-898,7.基金项目
西北农林科技大学青年学术骨干项目(E111020901) （E111020901）
教育部新世纪优秀人才支持计划(NCET-07-0701) （NCET-07-0701）
