基础医学与临床2011，Vol.31Issue(7)：767-772,6.
姜黄素通过下调IκBα磷酸化抑制食管鳞癌细胞的体外增殖
The effect of curcumin through suppression of IκBα phosphorylation on inhibition of proliferation of esophageal squamous cell carcinoma cell lines
摘要
Abstract
Objective To detect whether curcumin inhibits NF-κB signaling pathway through suppression IκBαt phosphorylation and evaluate the effects on proliferation and cell cycle in EC9706 and Ecal09 cell lines. Methods ESCC cells were treated with different concentrations of curcumin and viable cells were determined by MTT. Cytoplasmic extracts or whole cell extracts were examined for the expression of pIκBαt and cyclin D1. ESCC cells were treated with curcumin, 5-FU or combinations thereof for 72 h. Results Curcumin inhibited ESCC cells growth in a concentration-dependent manner. Curcumin inhibited IκBα phosphorylation and downregulated the expression of cyclin D1 in the two ESCC cell lines in a time-dependent manner. Cyclin D1 was declined in the forepart of curcumin-treated cells. Curcumin-treated cells increased in the Go/G1 phase and decreased in S phase. Conclusion Curcumin inhibits the phosphorylation of IκBct and downregulates the activation of NF-κB signaling pathway,leading to the suppression of proliferation in ESCC cell.关键词
食管鳞癌/姜黄素/NF-κB/IκBα/cyclin D1Key words
esophageal squamous cell carcinoma/ curcumin/ NF-kappaB/ IkappaBα/ cyclin D1分类
医药卫生引用本文复制引用
田芳,柴玉荣,江亚南,张晓艳..姜黄素通过下调IκBα磷酸化抑制食管鳞癌细胞的体外增殖[J].基础医学与临床,2011,31(7):767-772,6.基金项目
河南省医学科技攻关项目(200803005) （200803005）
郑州市科技攻关项目(0910SGYS33389-7) （0910SGYS33389-7）
