广东药学院学报2011，Vol.27Issue(3)：253-255,3.DOI:10.3969/j.issn.1006-8783.2011.03.010
巴戟天及其伪品的psbA-trnH序列鉴定
Molecular identification of psbA-trnH intergenic spacers' sequences of Morinda officinalis How and its counterfeit species
摘要
Abstract
Objective To perform the molecular identification for Morinda officinalis and its counterfeit species based on psbA-trnH intergenic spacers' sequences. Methods Total DNA was extracted and gene sequence was amplified by PCR. PCR product was directly sequenced after purification. Sequences were analyzed with Clustral X and MEGA 3.1 software. Results The length of psbA-trnH intergenic spacers'sequences was 306 bp. Distinctive variation sites among them can be observed. Conclusion The psbA-trnH intergenic spacers' sequences could be used to identificate Morinda officinalis and its counterfeit species.关键词
巴戟天/伪品/psbA-trnH序列/鉴定Key words
Morinda officinalis How/ counterfeit species /psbA-trnH intergenic spacers/ identification分类
医药卫生引用本文复制引用
姬生国,王东,张春荣,张宏意..巴戟天及其伪品的psbA-trnH序列鉴定[J].广东药学院学报,2011,27(3):253-255,3.基金项目
广东药学院博士启动基金项目(2007ZYX09) （2007ZYX09）
