重庆医学2011，Vol.40Issue(24)：2393-2394,2397,封2,4.DOI:10.3969/j.issn.1671-8348.2011.24.001
d-δ-三烯生育酚对人鼻咽癌CNE2细胞株增殖和凋亡的影响
Effect of d-δ-tocotrienols on the proliferation and apoptosis of nasopharyngeal carcinoma CNE2 cell line
摘要
Abstract
Objective To investigate effect of d-δ-tocotrienols on proliferation and apoptosis of nasopharyngeal carcinoma.Methods The experiment was carried out with control group and experimental group which included three different concentration of dδ-tocotrienols(40,50,60 μmol/L).Cytomorphology was analyzed by using Hoechst 33 258 fluorescent staining and microscopy.MTT experiment and flow cytometry were used to analyze the effect of d-δ-tocotrienols on proliferation,apoptosis and cell cycle of nasopharyngeal carcinoma cell line CNE2.Results After treated CNE2 cells with d-δ-tocotrienols for 48h, cell growth suppression rate rose obviously with the increase of d-δ-tocotrienols concentration, respectively be (27.71± 1.01)％ , (57.09±0.56)％ and (95.04±0.17)％ ,the differences were significant when compared with the control group (P＜0.01).Apoptotic cells with nucleus and cytoplasma compact dyeing or thick strip massive fluorescence increased obviously, the apoptotic cell rates were (9.18±1.86)％ , (34.97±1.01) ％ and (52.97±2.77)％ respectively in experimental group, compared with the control group (1.58±0.48)％ ,cell apoptosis rate significantly increased,the differences were significantly (P＜0.01).The percentage of S phase cells were (25.40±1.93)％ , (35.83±3.74)％ and ( 47.13±2.49)％ respectively in experimental group, cells were stopped at S phase,compared with control group (19.63±1.46)％ ,the differences were statistically significant (P＜0.01).Conclusion d-δ-atocotrien-ols has biological activity of anti-nasopharyngeal carcinoma CNE2 cell line.关键词
生育酚类/鼻咽肿瘤/细胞增殖/细胞凋亡Key words
tocopherols / nasopharyngeal neoplasms / cell proliferation / apoptosis引用本文复制引用
范才文,王建红,雷迅,易世江,肖胜军,曾攀,Huanbiao Mo,向秋..d-δ-三烯生育酚对人鼻咽癌CNE2细胞株增殖和凋亡的影响[J].重庆医学,2011,40(24):2393-2394,2397,封2,4.基金项目
广西高等学校优秀人才资助计划项目 （）
广西自然科学基金(2010GXNSFC013024). （2010GXNSFC013024）
