重庆医学2011，Vol.40Issue(24)：2401-2403,3.DOI:10.3969/j.issn.1671-8348.2011.24.004
NADPH氧化酶4在AGEs诱导内皮细胞活性氧生成中的作用研究
Role of NADPH oxidase 4 in generation of intracellular ROS in endothelial cells stimulated by AGEs
摘要
Abstract
Objective Advanced glycation end products (AGEs) can induce the intracellular generation of reactive oxygen species (ROS) and cause the dysfunction of endothelial cells.This may accelerate development of vascular atherosclerosis.We investigated the role of NADPH oxidase 4 ( Nox4) in the generation of intracellular ROS in endothelial cells stimulated by AGEs.Methods Human umbilical vein endothelial cells (HUVECs) were cultured and incubated with AGEs and intracellular ROS was measured with 2' ,7'-dichlorodihydrofluorescein diacetate (DCF-DA).siRNA was used to silence Nox4.The role of Nox4 in the generation of ROS was observed.Results After AGEs stimulation,ROS in HUVECs was significantly elevated,while Nox4 siRNA transfection inhibited the generation of ROS significantly.Nox4 siRNA transfection reduced ROS at basal level by 45.9％ , and significantly inhibited AGEs- induced ROS elevation.Conclusion Altogether,the results demonstrate that Nox4 is the major source of intracellular ROS in endothelial cells,and indicate potential targets for the inhibition of the atherogenic signals triggered by AGEs.关键词
糖基化产物,高级/内皮细胞/活性氧/NADPH氧化酶Key words
glycosvlation end products , advanced : endotheltal cells : reactive oxygen species / NADPH oxidase引用本文复制引用
刘洪彬,于世勇..NADPH氧化酶4在AGEs诱导内皮细胞活性氧生成中的作用研究[J].重庆医学,2011,40(24):2401-2403,3.基金项目
国家自然科学基金资助项目(No.81000134). （No.81000134）
