电化学2011，Vol.17Issue(2)：180-185,6.
基于锁核酸探针的传感器用于BCR/ABL融合基因检测的研究
Hairpin Locked Nucleic Acids Probe Based Electrochemical Biosensor for Detection of BCR/ABL Fusion Gene
摘要
Abstract
In this article ,a new type of structure hairpin locked nucleic acids(LNA) probe was designed to detect BCR/ABL fusion gene in Chronic Myelocytic Leukemia. The LNA probe was immobilized on the gold electrode(AuE) through sulfur-Au interaction to construct specific electrochemical biosensor. The electrochemical response of the sensor to hybridization of the LNA probe with the target DNA was studied using [ Cu( R)2]2+ as an electrochemical indicator. The optimal condition was discussed. The experimental results indicated that in pH 7.4 PBS buffer solution, this new method has excellent selectivity for single base mismatch and complementary after hybridization. The linear relationship between the increased oxidation peak current of [ Cu(R) 2 ] 2+ and the concentration of complementary strand was observed in the range of 1. 0 × 10-8 ～ 1.0 × 10-6 mol/L. The detection limit was 2.0 × 10 -9 mol/L.关键词
发夹锁核酸探针/生物传感器/BCR/ABL融合基因/苯甲酸二聚铜配合物Key words
hairpin LNA probe/ electrochemical biosensor/ BCR/ABL fusion gene/ [ Cu2 (C7H5O2)4(C2H6O)2]分类
化学化工引用本文复制引用
汪红梅,林丽清,翁少煌,王丽满,刘银环,林新华..基于锁核酸探针的传感器用于BCR/ABL融合基因检测的研究[J].电化学,2011,17(2):180-185,6.基金项目
国家自然科学基金项目(20675015),国家863计划(No.2008AA02Z433),福建省自然科学基金(No.2010J06011,2010J01032),福建省教育厅(No.JA10155),福建省高校产学研科技重点项目(2010Y4003)资助 （20675015）
