中国药理学与毒理学杂志2011，Vol.25Issue(4)：344-348,5.DOI:10.3867/j.issn.1000-3002.2011.04.003
NCS-382对丙泊酚、氯胺酮和依托咪酯催眠镇痛作用的影响
Effects of NCS-382 on hypnosis and analgesia of propofol, ketamine and etomidate in mice
摘要
Abstract
OBJECTIVE To investigate the effect of NCS-382 on hypnosis and analgesia of propofol, ketamine and etomidate in mice. METHODS ① Analeptic test: a total of 120 mice were ip given propofol 200 mg·kg -1 , ketamine 150 mg·kg -1 and etomidate 40 mg·kg -1 to establish a hypnosis model, respectively. Then, the mice were icv given aCSF and NCS-382 1, 5 and 25 μg· kg-1, respectively, and sleeping time was recorded. ② Hot-plate test: a total of 80 mice were ip given normal saline 10 ml· kg - 1 and ketamine 40 mg· kg - 1, respectively, and were ith given aCSF and NCS-382 1,5 and 25 μg·kg-1, respectively. The pain threshold in hot-plate test (HPPT) was recorded. ③ Writhing test: a total of 80 mice were respectively ip given normal saline 10 ml·kg-1 and ketamine 40 mg·kg-1. Then, the mice were ith given aCSF and NCS-382 1, 5 and 25 μg·kg-1, respectively. They were ip given 1.0％ acetic acid 10 ml·kg-1, then writhing time within 15 min were recorded. RESULTS ① Analeptic test: the sleeping time in NCS-382 1.5 and 25 μg·kg-1 groups was significantly shortened by propofol for (81 ±30)min to 44 ±23, 40 ±21 and (40 ± 17 ) min, by ketamine for ( 19 ± 4 ) min to 8 ± 4, 12 ± 3 and ( 15 ± 4 ) min; by etomidate for (31 ±11)min to 21 ±9, 22 ±6 and (23 ± 10)min. ② Hot-plate test: compared with self basal HPPT, ketamine could significantly prolong HPPT 10 -30 min after administration (P ＜ 0.05 ). NCS-382 had no effect on the prolonged HPPT by ketamine. ③ Writhing test: compared with normal control group, NCS-382 had no effect on writhing times. The writhing times in ketamine groups decreased significantly. NCS-382 had no effect on the increased writhing times induced by ketamine. CONCLUSION NCS-382 may mediate the hypnotic effect of propofol, ketamine and etomidate, but it may have little analgesic effect as ketamine does.关键词
受体/γ-羟基丁酸/NCS-382/丙泊酚/氯胺酮/依托咪酯/催眠/镇痛Key words
receptor/ gamma-hydroxybutyrate/ NCS-382/ propofol/ ketamine/ etomidate/hypnosis/ analgesia分类
医药卫生引用本文复制引用
宋苏沛,王雷,廖艺聪,刘晶晶,张明阳,周美艳,周慧轩,唐然,戴体俊..NCS-382对丙泊酚、氯胺酮和依托咪酯催眠镇痛作用的影响[J].中国药理学与毒理学杂志,2011,25(4):344-348,5.基金项目
国家自然科学基金资助项目(30471657) （30471657）
国家自然科学基金资助项(30872432) （30872432）
国家自然科学基金资助项目(39970715) （39970715）
江苏省自然科学基金资助项目(BK2001143) （BK2001143）
