广东牙病防治2011，Vol.19Issue(7)：339-342,4.
飞行时间质谱法检测干扰素调节因子6单核苷酸多态性的研究
Fast and High-throughput Genotyping of SNPs of Cleft Related Gene IRF6 Using MALDI-TOF
摘要
Abstract
Objective To establish a high throughput, fast and accurate method for genotyping of SNPs of cleft related gene IRF6. Methods IRF6 gene fragment including rs2235371 and rs642961 was amplified and purified as the template of the following primer extension reaction. The single base extension reaction was carried out using SNP specific extension primer. The products were purified and analyzed by MALDI-TOF. Results The method for fast and high throughput genotyping rs2235371 and rs642961 has been set up. The frequencies of rs2235371 and rs642961 in our 201 unrelated healthy Han Chinese using genotyping by MALDI-TOF are as same as reported frequencies of Han Chinese in HapMap. Conclusion MALDI-TOF is a fast, high throughput and accurate method for genotyping, and is suitable for large sample cleft related genes screening.关键词
基质辅助激光解吸电离飞行时间质谱技术/干扰素调节因子6/单核苷酸多态性/唇腭裂Key words
MALDI-TOF-MS/ Interferon regulatory factor 6/ Single nucleotide polymorphisms/ Cleft lip and palate分类
医药卫生引用本文复制引用
许铭炎,邓小玲,陈锡和,陈庆珊,姚小武,唐世杰..飞行时间质谱法检测干扰素调节因子6单核苷酸多态性的研究[J].广东牙病防治,2011,19(7):339-342,4.基金项目
"重生行动-全国贫困家庭唇腭裂儿童手术康复计划"项目 （）
