中国医疗设备2011，Vol.26Issue(8)：21-24,4.DOI:10.3969/j.issn.1674-1633.2011.08.007
开腹及超声引导下深植入法和浅植入法建立兔肝VX2肿瘤模型的比较
Comparative Study of Rabbit Liver VX2 Tumor Model Made by Implanting Tumor Tissue Mass and Its Suspension into Deep and Superficial Liver Parenchyma under Laparotomy and Ultrasound-guided
摘要
Abstract
Objective To study success rate and ectopic implantation rate of rabbit liver VX2 tumor model made by implanting tumor tissue mass and its suspension into deep and superficial liver parenchyma under laparotomy and ultrasound- guided. Methods 80 New Zealand rabbits were divided into eight groups (each group 10) according to implanting tissue mass or its suspensions and implanting depth. The VX2 tumor tissue mass or its suspensions were implanted into rabbits liver under laparotomy and ultrasound-guided(superficial group depths≤20mm, deep group depth ＞ 20mm), 1W, 2W, 3W after implantation, ultrasound examination was done respectively, and after 3W rabbits were sacrificed to observe the tumor size in situ, dystopia and made pathological examination. Results The success rate of rabbits liver VX2 tumor model made by two methods were 100％. Tumor volume was significantly greater in group of tissue mass than in group of suspensions ( P ＜ 0.05 ) .In tissue mass deep implantation group, there was no case of ectopic implantation,. In tissue mass superficial implantation group,there was 1 case of abdominal implantation under ultrasound- guide, there is no case of abdominal implantation under laparotomy. In tissue mass suspension deep implantation group, there were 2 cases of abdominal implantation under ultrasound-guided, there was no case of abdominal implantation under laparotomy. In tissue mass suspension superficial implantation group, there were 2 cases of abdominal implantation under laparotomy, there were 5 cases of abdominal implantation under ultrasound- guided, among them there was 1 case of abdominal and abdominal wall implantation. Conclusion The method of rabbit liver VX2 tumor model made by implanting tumor tissue mass and its suspension into rabbit liver under ultrasound-guided is simple, minimally invasive and highly successful. The method of tumor tissue mass implantation is superior to that of tissue mass suspensions, and that of deep implantation is superior to that of shallow implantation关键词
彩色多普勒超声诊断仪/超声检查/肝肿瘤/动物模型/兔肝VX2肿瘤模型Key words
ultrasonography/ liver neoplasm/ implantation/ animal model分类
医药卫生引用本文复制引用
陈松旺,周云,孟凡荣,赵美丽..开腹及超声引导下深植入法和浅植入法建立兔肝VX2肿瘤模型的比较[J].中国医疗设备,2011,26(8):21-24,4.基金项目
南京医科大学科技发展基金项目(06NMUZ038). （06NMUZ038）
