山东医药2011，Vol.51Issue(26)：29-31,3.
携带Foxp3基因慢病毒载体的构建
Construction of lentiviral vector carrying Foxp3 gene
摘要
Abstract
Objective To construct lentiviral vector carrying Foxp3 gene and packaged in lentivrial particles ( PWPXLMOD-Foxp3), making the basis for obtaining CD4+ CD25+ regulatory T cells in vitro.Methods The PWPXL-MOD-Foxp3 expression vector was be constructed by double digests.The recombinant lentiviral vector and else four packaging plasmids were co-transfected into human embroic kidney 293T cells by using calcium phosuhate technique.The supernatant was collected, purified, concentrated, and then, the viral titers were tested by flow cytometer.Result The titer of the lentiviral particles was 3.3×108 IU/ml.Conclusions The high-titer lentvirus vector containing Foxp3 geng is constructed successfully, which will contribute to obtain CD4+ CD25+ regulatory T cells in vitro.关键词
叉头样转录因子p3基因/慢病毒/CD4+CD25+调节性T细胞/基因治疗Key words
forkhead box P3/ lentiviral/ CD4+ CD25+ regulatory T cells/ geng therapy分类
医药卫生引用本文复制引用
陈春,李永翔,罗庆礼..携带Foxp3基因慢病毒载体的构建[J].山东医药,2011,51(26):29-31,3.基金项目
国家自然科学基金资助项目(30871207) （30871207）
安徽省科技厅重点科研计划项目(7020304101) （7020304101）
安徽省教育厅重点科研项目(KJ2008A154). （KJ2008A154）
