实验动物与比较医学2011，Vol.31Issue(4)：269-272,4.DOI:10.3969/j.issn.1674-5817.2011.04.009
nklin大肠杆菌ompT基因的相对表达定量PCR方法的建立
Detection of Relative Expression of ompT Gene in Escherichia coli by SYBR Green I RT-PCR
摘要
Abstract
Objective To establish a method to relatively quantify the expression of ompT and the gene of APEC strain E058 in vivo. Methods Two-step real time quantitative RT-PCRs (qRT-PCR) of ompT and gapA were developed based on SYBR Green I, respectively. In these qRT-PCRs, ompT was identified as the target gene and gapA as internal reference, and two standard curves were established using a series dilution of cDNA synthesized from the RNA of APEC E058 grown statically to exponential phase in rich medium. Results qRT-PCR of ompT was established. In chicken challenge model, the expressions of ompT in APEC E058 were up-regulated 6.69-fold compared to that of APEC E058 grown statically to exponential phase in rich medium. Conclusion The established qRT-PCR was reliable for the expression analysis of ompT.关键词
SYBR GreenI/定量反转录PCR/ompT/表达Key words
SYBR G-reenI/ qRT-PCR/ ompT/ expression分类
生物科学引用本文复制引用
徐晓静,殷为民,赵李祥..nklin大肠杆菌ompT基因的相对表达定量PCR方法的建立[J].实验动物与比较医学,2011,31(4):269-272,4.
