中国畜牧兽医2011，Vol.38Issue(6)：83-86,4.
流产型布鲁氏菌Omp22基因酵母双杂交诱饵载体的构建及鉴定
Construction and Identification of Bait Vector Containing Omp22 Gene from Brucella abortus in Yeast Two Hybrid System
李向阳 1张嘉保2
作者信息
- 1. 吉林大学农学部畜牧兽医学院,吉林长春130062
- 2. 内蒙古民族大学成人教育学院,内蒙古通辽028043
- 折叠
摘要
Abstract
The coding region of Omp22 gene from Brucella abortus was amplified by PCR and then fused with pSOS vector.After confirmation with sequence analysis, the plasmid was transformed into the yeast cell cdc25H, the toxicity and transcriptional auto activation of expressed protein were tested. The results indicated that the coding region was successfully amplified and subcloned into pSOS vector, and the cdc25H transformed with bait plasmid grew well on SD/glucose (-L) plate. These data showed that the bait vectors pSOS-Omp22 was expressed correctly without toxicity and could not auto activate. The pSOS-Omp22 could be applied for the screening of proteins interacting with the Omp22 in macrophages cDNA library in the yeast two hybrid systems.关键词
酵母双杂交系统/Omp22基因/诱饵载体/自激活Key words
yeast two hybrid system/ Omp22 gene/ bait vector/ self-activation分类
农业科技引用本文复制引用
李向阳,张嘉保..流产型布鲁氏菌Omp22基因酵母双杂交诱饵载体的构建及鉴定[J].中国畜牧兽医,2011,38(6):83-86,4.
