中国畜牧兽医2011，Vol.38Issue(6)：136-141,6.
猪繁殖与呼吸综合征RT-LAMP检测方法研究
Development of RT-LAMP for Detecting Porcine Reproductive and Respiratory Syndrome Virus
摘要
Abstract
Applying reverse transcription LAMP (RT-LAMP), according to the N protein conserved sequence of PRRSV ORF5 genes from six regions, two sets of primers were designed. By optimizing reaction system and conditions, the RT-LAMP method for detecting PRRSV was developed after sensitivity and specificity tests. The research results indicate that PRRSV nucleic acids amplification can be completed within 1 h in the isothermy condition of 63 ℃ only using a common constant temperature water-bath, test results can be judged with naked eyes after adding nucleic acids dye SYBR Green Ⅰ . It provided a kind of cheap, simple, speedy, high sensitivity and specificity PRRSV detecting method for work field and grass-roots units.关键词
猪繁殖与呼吸综合征病毒/RT-LAMP/检测方法/研究Key words
porcine reproductive and respiratory syndrome virus/RT-LAMP/detection method / research分类
农业科技引用本文复制引用
杨素,沙才华,顾海洋,董尚智,廖秀云,徐海聂,薄清如,罗宝正,陈伟生..猪繁殖与呼吸综合征RT-LAMP检测方法研究[J].中国畜牧兽医,2011,38(6):136-141,6.基金项目
珠海市科技计划项目(PC20081079). （PC20081079）
