中国畜牧兽医2011，Vol.38Issue(6)：218-221,4.
几种检测猪弓形虫病ELISA诊断试剂盒的对比
Comparison of Five ELISA Kits for Detection of Anti-Toxoplasma gondii Antibodies in Experimentally Infected Swine
摘要
Abstract
The aim of the present study was to evaluate and compare the sensitivities of five ELISA kits for detection of Toxoplasma gondii infection in experimentally infected pigs. The PCR-positive serum samples from experimentally infected pigs were examined by the five ELISA kits. The results of different ELISA kits were compared among them and with that of PCR amplification to evaluate and compare their sensitivities. One ELISA kit (ELISA-A) made in China showed 100％ detection of T. gondii antibody in experimentally infected pigs, showing 100％ sensitivity. Whereas, the other EL1SA kits displayed different low sensitivities. These results indicated that the ELISA-A kit is the most sensitive for detection of T. gondii infection in pigs.关键词
弓形虫/猪弓形虫病/人工感染/ELISA/对比Key words
Toroplasma gondii / swine toxoplasmosis / experimental infection/ ElISA/ comparison分类
农业科技引用本文复制引用
何勇,朱艳平,袁子国,尹创成,周东辉,林瑞庆,朱兴全,翁亚彪..几种检测猪弓形虫病ELISA诊断试剂盒的对比[J].中国畜牧兽医,2011,38(6):218-221,4.基金项目
国家自然科学基金项目(30901067) （30901067）
高等学校博士学科点专项科研基金(20094404120016) （20094404120016）
广东省科技计划项目(2009B020307011) （2009B020307011）
广东省自然科学基金(10451064201005443) （10451064201005443）
广东省高等学校自然科学基金(9451064201003715) （9451064201003715）
畜疫病病原生物学国家重点实验室(SKLVEB2009KFKT014 （）
SKLVEB2010) （）
教育部"长江学者和创新团队发展计划"创新团队项目(IRT0723)资助. （IRT0723）
