生殖医学杂志2011，Vol.20Issue(4)：313-318,6.DOI:10.3969/j.issn.1004-3845.2011.04.015
人卵巢黄素化颗粒细胞细胞周期蛋白D2的表达与卵巢功能及反应性的相关性研究
Expression of cyclin D2 in human luteinized granulosa cells and its correlation with ovarian function
摘要
Abstract
Objective.. To investigate the correlation of cyclin D2 expression in human luteinized granulosa cells with ovarian function and ovarian response.Methods: Ovarian luteinized granulosa cells were collected from 48 women who underwent in vitro fertilization programme in this department. According to the number of follicles ≥16 mm obtained during oocyte retrieval, the patients were divided into three groups: high response group (n = 12), moderate response group (n=30) and poor response group (n=6). Immunocytochemistry was performed to detect the expression of cyclin D2 protein and PCNA protein in human luteinized granulosa cells. The cyclin D2 mRNA in granulosa cells was determined by reverse transcriptase-polymerase chain reaction (RT-PCR)technique. Serum concentrations of hormones were measured by chemiluminescence immunoassay (CLIA).Results: (1)Expression of cyclin D2 protein in human luteinized granulosa cells was negatively correlated with age(r=-0. 547, P＜0. 01), serum baseline FSH (r=-0. 456, P＜0.05) and the ampules of gonadotropins used (r=-0. 451, P＜0. 05), while positively correlated with the number of oocytes retrieved and serum estradiol (E2) levels at the day of hCG administration (r=0. 592, P＜0.01 and r=0. 626, P＜0.01), respectively. The expressions of cyclin D2 protein were significantly different among three groups (F=-4. 995, P＜0. 05). (2)Expression of cyclin D2 mRNA in human luteinized granulosa cells showed a negative correlation with age (r=-0. 510,P＜0.05) and serum baseline FSH (r=-0. 891,P＜0.01), and a positive correlation with number of oocytes retrieved (r=0. 629, P＜0.01) and serum E2 levels at the day of hCG administration (r=0.524, P＜0. 05); cyclin D2 mRNA expression showed a significant difference among different ovarian responders (F = 8. 843, P ＜ 0.01 ). (3) There was a significant positive correlation of cyclin D2 mRNA (r = 0. 498, P ＜ 0.05) and protein expression (r =0. 696, P＜0. 01) with proliferating cell nuclear antigen labeling index in luteinized granulosa cells.Conclusions: The expression of cyclin D2 in human luteinized granulosa cells may serve as an indicator of ovarian reserve function and ovarian response. The results provide supporting evidence for the importance of cyclin D2 in granulosa cell proliferation and follicle development.关键词
颗粒细胞/细胞周期蛋白D2/细胞增殖/卵巢功能/卵巢反应性Key words
Granulosa cells/ Cyclin D2/ Cell proliferation/ Ovarian function/ Ovarian response引用本文复制引用
崔娜,郝桂敏,赵志明,徐素欣,曹金凤,王玮..人卵巢黄素化颗粒细胞细胞周期蛋白D2的表达与卵巢功能及反应性的相关性研究[J].生殖医学杂志,2011,20(4):313-318,6.基金项目
河北省卫生厅科研基金项目(20100055) （20100055）
