化工学报2011，Vol.62Issue(7)：2013-2019,7.DOI:10.3969/j.issn.0438-1157.2011.07.034
铜绿假单胞菌WJ-1降解原油特性
Oil-degradation characteristics of Pseudomonas aeruginosa WJ-1
摘要
Abstract
A hydrocarbon-degrading facultative strain WJ-1 was isolated from the formation water of Menggulin oil reservoir and identified as Pseudomonas aeruginosa by the physiological-biochemical tests and 16S rDNA technology. In order to investigate the biodegradation characteristics of this strain with crude oil as sole carbon source, four different crude oils gathered from Xinjiang, Zhongchakou, DaqingJiangqiao, Menggulin oil reservoirs were used. The biodegradation effect on four fractions (saturated hydrocarbons, non-hydrocarbons, aromatics, asphaltene and resin) of oil were analyzed by rod thin layer chromatography and the results showed that the relative contents of non-hydrocarbons, asphaltene and resin decreased in average by above 10％ after WJ-1 strain metabolism. Gas chromatography experiments indicated that the light components of saturated hydrocarbons increased, the heavy components of saturated hydrocarbons and phenanthrene of aromatics decreased. The viscosity and solidification point of four crude oils in average reduced by about 45％ and 6℃. Physical simulation studies using four different crude oils in porous media showed that the strain WJ-1 could enhance oil recovery by 4.78％, 5.15％,7.39％, 9.02％ respectively in contrast to water flooding.关键词
铜绿假单胞菌/原油降解/黏度/凝固点/物理模拟Key words
Pseudomonas aeruginosa/ hydrocarbon-degrading/ viscosity/ solidification point/ physical simulation分类
生物科学引用本文复制引用
夏文杰,董汉平,俞理,黄立信..铜绿假单胞菌WJ-1降解原油特性[J].化工学报,2011,62(7):2013-2019,7.基金项目
国家高技术研究发展计划项目(2009AA063504) （2009AA063504）
中国石油天然气股份有限公司科学研究与技术开发项目(2008A-1403) （2008A-1403）
国家重点基础研究发展计划项目(2005cb221308). （2005cb221308）
