中国人兽共患病学报2011，Vol.27Issue(7)：601-604,4.
埃博拉病毒科特迪瓦型核蛋白的原核表达及纯化
Prokaryotic expression and purification of nucleoprotein gene of Ebola virus C(o)te d'Ivoire type
摘要
Abstract
To obtain recombinant protein of the nucleoprotein (NP) of Ebola virus C(o)te d'Ivoire type using prokaryotic expression system, the synthesized NP gene was sub-cloned to generate pET-28 (a)-C-NP recombinant plasmid, which was then transformed into E.coli BL21 (DE3) competent bacteria.The expression of NP recombinant protein was induced by IPTG and analyzed by SDS-PAGE.The expressed protein was purified using His-Band Ni+ affinity chromatography and identified by Western blot and protein sequencing.The recombinant expression plasmid pET-28 (a)-C-NP was obtained and the recombinant NP protein was expressed and purified.The obtained NP protein was confirmed by Western blot and protein sequencing.These results demonstrate that the NP recombinant protein was expressed and characterized.In conclusion, successfully expressed and purified Ebola virus nucleoprotein type C(o)te d'Ivoire aid the foundation for the follow-up study.关键词
埃博拉病毒/NP蛋白/原核表达/蛋白纯化Key words
Ebola virus/ NP protein/ prokaryotic expression/ protein purification分类
医药卫生引用本文复制引用
王雪敏,王皓婷,邱亚峰,史子学,邵东华,王水明,刘学辉,王志亮,马志永..埃博拉病毒科特迪瓦型核蛋白的原核表达及纯化[J].中国人兽共患病学报,2011,27(7):601-604,4.基金项目
公益性行业(农业)科研专项(200903037-06)和江苏出入境检验检疫局项目(2011KJ03)资助 （农业）
