中国人兽共患病学报2011，Vol.27Issue(7)：629-633,5.
用聚合酶链反应技术对粉尘螨和屋尘螨进行鉴别和检测
Identification and detection of Dermatophagoides farina and Dermatophagoides pteronyssinus by Polymerase Chain Reaction
摘要
Abstract
The purpose of the present study was to identify and detect Dermatophagoides farina (D.f) and Dermatophagoides pteronyssinus (D.p)by Polymerase Chain Reaction (PCR) technology.A randomly primed PCR (RP-PCR) technology was used to amplify the DNA of D.f and D.p, and three amplified bands were selected for DNA sequencing and analysis.According to the resuLts of DNA sequencing, another two pairs of primers were designed to identify and detect D.f and D.p,then the specificity and sensitivity of these primers were analyzed.In amplification of RP-PCR, two significant bands around 500bp and 750bp were observed in amplified products of D.f, while only a clear band about 500bp in D.p and no product larger than 500bp appeared.Three new DNA fragments of D.f and D.p were sequenced and analyzed in homology.As the new primers designed were highly specific and sensitive in detection of the two mites, RP-PCR technology could be used to identify D.f and D.p.Results indicate that the PCR method verified in the research has a potentiality to detect D.f and D.p in the environment.关键词
粉尘螨/屋尘螨/PCRKey words
Dermatophagoides farinae / Dermatophagoides pteronyssinus / PCR分类
医药卫生引用本文复制引用
丁雪,罗晓东,罗洁琳,李小敏,沈浩贤,陈代雄..用聚合酶链反应技术对粉尘螨和屋尘螨进行鉴别和检测[J].中国人兽共患病学报,2011,27(7):629-633,5.基金项目
广州市教育局科技计划项目赞助(08A098) （08A098）
广东省自然基金资助项目(06022684) （06022684）
