中国人兽共患病学报2011，Vol.27Issue(7)：597-600,609,5.
日本血吸虫感染鼠巨噬细胞IL-13Rα2的表达
Expression on IL-13Rα2 in macrophages of Schistosoma japonicum-infected mice
摘要
Abstract
The purpose of this study was to investigate the expression on intracellular proteins of IL-13Rα2 in the macrophages of Schistosomiasis japonicum mice, and to offer the experiment foundation for elucidating IL-13 responding cell and mechanism of fibrogenesis in Th2-mediated diseases. A large number of mice were exposed to cercariae and established S. japonicum mouse model. IL-13Rα2 expressed in the granulomas of liver tissues and peritoneal macrophages was determined by immunofluorescent labeling with primary antibody of goat anti-mouse IL-13Rα2 antibody and monoclonal anti-CD68 antibody,respectively. Coffee bean-like IL-13Rα2 positive staining was showed in the hepatic granuloma in fluorescentmicroscopy. Co-expression of IL-13Rα2 and CD68 protein in peritoneal macrophages from 8-week infected mice was observed. It exhibited the distribution of IL-13Rα2 in cytoplasm concentrated on the inner layer of membrane in macrophages. It's suggested that IL-13Rα2 expressing macrophages might be a critical contributor to the immunopathogenesis of schistosomiasis.关键词
血吸虫病/肉芽肿/巨噬细胞/IL-13Rα2/CD68/免疫荧光染色技术Key words
schistosomiasis/ granuloma/ macrophages/ IL-13Rα2/ CD68/ immunofluorescence staining technique分类
医药卫生引用本文复制引用
王维,郑胜生,祁瑶,闻惠琴,刘丽丽,沈继龙..日本血吸虫感染鼠巨噬细胞IL-13Rα2的表达[J].中国人兽共患病学报,2011,27(7):597-600,609,5.基金项目
国家自然科学主任基金项目(30940061),安徽省高等学校省级自然科学研究计划重点项目(KJ2009A80)和安徽医科大学博士启动基金(XJ201012)联合资助 （30940061）
