宁夏大学学报（自然科学版）2011，Vol.32Issue(2)：164-167,4.
SHV-12型超广谱β-内酰胺酶的原核表达及免疫原性分析
Prokaryotic Expression of SHV-12 Type ESBLs and Immunogenicity Analysis
摘要
Abstract
SHV-12 ESBLs target gene was amplified by PeR and subcloned into a vector pET-28a(+).The positive plasmid contained SHV-12 ESBLs gene was transferred into BL21(DE3) and was induced to expression and purification by IPTG.It proved that the SHV-12 ESBLs was expressed and purified as a molecular weight 30 KDa fusion protein which was verified by SDS-PAGE.Western blotting of the results showed that there was specific band.Nitrocefin colour reaction certificated that there was dynamic ESBLs.Inclusion body can stimulate Balb/c mice to produce high titer antibodies to 1∶106, which the recombinant ESBLs can be used as immune with good irnrnunogenicity.关键词
克隆与表达/超广谱β-内酰胺酶(ESBLs)/抗体效价Key words
cloning and expression/ extended-spectrum β-lactamases(ESBLs)/ antibody titer分类
生物科学引用本文复制引用
贾芳,周学章..SHV-12型超广谱β-内酰胺酶的原核表达及免疫原性分析[J].宁夏大学学报（自然科学版）,2011,32(2):164-167,4.基金项目
国家自然科学基金资助项目(31060348) （31060348）
宁夏科技攻关基金资助项目(KGZ-12-09-06) （KGZ-12-09-06）
