实用肿瘤杂志2011，Vol.26Issue(4)：351-355,5.
视黄酸对放射性肺损伤大鼠肺组织CD36表达的影响
Effect of retinoic acid on expression of CD36 in lung tissue of rats with radiation-induced lung injury
摘要
Abstract
Objective To investigate the effect of retinoic acid (RA) on the expression of CD36 in lung tissue of rats with radiation-induced lung injury.Methods Eighty Wistar rats were randomly divided into four groups, with 20 rats in each group,including control group( A), RA treatment group(B), radiation group ( C), and radiation with RA treatment group ( D ).Rats in groups C and D were radiated with 6 MV-X ray of linear accelerator at a dose of 15 Gy,rats in groups B and D were given with 20 mg/( kg · d)RA by gastrogavnge.One,2,4 and 8 week after radiation samples of bronchoalveolar lavage fluids (BALF) and lung tissue were taken for HE stain, Massion stain and immunohistochemistry stain to detect the expression of CD36.Results HE and Massion staining showed inflammatory ce]is exudation in the alveolar spaces 1 week after radiation ,followed by interstitial edema and destroyed structure and collagen fibers appearing in pulmonary interstitial at 4 and 8 week.No significant difference of pathology was seen between group A and B at all time points, while the rat pulmonary inflammation and edema alleviated, with decreased collagen fibers in group D compared to group C.The number of cells in BALF increased significantly in group C compared to group A at all time points( P ＜ 0.01 ), while decreased in group D compared to group C especially at week 4 and 8 ( P ＜ 0.005 ).There was no difference in CD36 expression between group A and B at all time points,while it was enhanced significantly in group C and group D at week 1,2,4 and 8 compared to group A (P ＜ 0.0001).The expression of CD36 was weakened in group D compared to group C, especially at week 4 and 8.Conclusion The expression of CD36 is increased significantly in lung tissue of rats with radiation-induced lung injury, and refinoic acid can inhibit CD36 expression, which suggests RA might be used to preventiun and treatment of radiation-induced lung injury.关键词
肺肿瘤/辐射损伤/维甲酸/药理学/支气管肺泡灌洗液/抗原,CD36/代谢/肺泡/病理学/随机对照试验Key words
lung ncoplasms/ radiation injuries/ tretinoin/pharmacology/ bronchoal veohtr lavage fluid/ antigens, CD36/metabolism/ pulmonary alvcoli/pathology/ randomized controlled trial分类
医药卫生引用本文复制引用
曹丽艳,赵俊华,赵玉霞,于洪,门桐林,赵娜,刘丹,白露..视黄酸对放射性肺损伤大鼠肺组织CD36表达的影响[J].实用肿瘤杂志,2011,26(4):351-355,5.基金项目
沈阳市社会事业科技创新项目资助(1081269-9-00) （1081269-9-00）
