林产化学与工业2011，Vol.31Issue(3)：39-44,6.
超声波辅助提取槐花总黄酮及其对酪氨酸酶的抑制作用
Ultrasound-assisted Extraction of Flavanoids from Sophora japonica L.and Its Inhibition on Tyrosinase
摘要
Abstract
Flavonoids ofSophorajapoaica L. were extracted with ultrasoued-assisted. The optimal technological conditions were determined by single-factor and orthogonal experiment. The inhibition of .S japonica L. extract on mushroom tyrosinase was studied by tinting the enzyme activities under the influence of extract. The results showed that the optimum extraction conditions were as follows: S. japonica L. powder 2.0 g, volume fraction 60 ％ ethanol 40 mL, extraction temperature 8O ℃, ultrasound power 3OO W and time 3O min. The final fiavonoids yield is ( 16.5 ± 0. 4 ) ％. The concentration of S. japonica L. extract was O. 03 g/L, when the inhibition on tyrosinase ( IC50 ) was 50 ％. It was about fiftienth more than that of arbutin ( IC50= 1.60 g/L ).The kinetics of inhibition on enzyme by S. japonica L. extract was the type of competitive inhibition.关键词
槐花总黄酮/超声波辅助提取/蘑菇酪氨酸酶/抑制作用Key words
flavanoids of Sophora japoaica L./ ultrasound-assisted extraction/ mushroom tyros inase/ inhibition分类
化学化工引用本文复制引用
杜志云,涂增清,张焜 ,莫容清,潘文龙,汤志恺..超声波辅助提取槐花总黄酮及其对酪氨酸酶的抑制作用[J].林产化学与工业,2011,31(3):39-44,6.基金项目
国家自然科学基金资助项目(21042003) （21042003）
广东省科技计划资助项目(2007A020300007-10) （2007A020300007-10）
