原子能科学技术2011，Vol.45Issue(6)：763-768,6.
微波辐射对苜蓿种子发芽及种带固氮菌固氮酶活性的影响
Effect of Microwave Irradiation on Alfalfa Seeds Germination and Nitrogenaseactivity of Endophytic Diazotrophs in Seeds
摘要
Abstract
Various microwave powers were used to irradiate alfalfa seeds with various time to study the effect of microwave irradiation on nitrogenase activity of endogenous azotobacter and germination of seeds.Germination rate, germination speed and nitrogenase activity of pure cultures that derived from seed-carried azotobacter were tested.The results indicate that: 800 W, 20 s and 500 W, 40 s are found with highest germination rate on the 1st day, which is 122％ and 88.9％ times higher than the control group (P＜0.01), and highest total germination rate is found within the 14-day period, which is 5.51％ and 3.35％ times higher than the control group, respectively.Under the two treatments, radical length on the 4th day is 29.8％ and 41.9％ times longer than the control group, and more sensitive nitrogenase activity is found on condition of various time than various powers.Short time treatments on condition of the two irradiation powers can increase nitrogenase activity conspicuously, and the treatments that treated more than 32 s make nitrogenase activity lower than the control group, conspicuously.Nitrogenase activity is found 104.90％ times higher than the control group on condition of 24 s.关键词
种子活力/微波辐射/乙炔还原法/固氮酶活性Key words
seeds vigor/ microwave irradiation/ ethylene reduction method/ nitrogenaseactivity分类
生物科学引用本文复制引用
张淑卿,李剑峰,师尚礼,霍平慧,朱新强,赵文翰,陶茸..微波辐射对苜蓿种子发芽及种带固氮菌固氮酶活性的影响[J].原子能科学技术,2011,45(6):763-768,6.基金项目
国家自然科学基金资助项目(31060326) （31060326）
"十一五"国家科技支撑项目资助(2007BAD52B06) （2007BAD52B06）
农业部行业专项资助项目(nyhyzx07-022) （nyhyzx07-022）
现代农业产业技术体系建设专项资助项目 （）
