临床肝胆病杂志2011，Vol.27Issue(8)：824-826,836,4.
大麻素受体1、FAK mRNA在小鼠肝纤维化形成过程中肝组织中的表达及相互关系
Hepatic expression of CB1 in rats with fibrosis and the relationship with FAK
摘要
Abstract
Objective To evaluate hepatic expression of CBl mRNA in rats during liver fibrogenesis and study the relationship between CB1 and FAK. Methods Liver fibrosis model was prepared by intraperitoneal injection of carbon tetrachloride ( 10％ ). Liver tissues and serum samples were collected at 2, 4, 6 and 8 week. The scores of fibrosis stage (S) were performed. The mRNA levels of CB1 mRNA and FAK were determined by quantitative RT - PCR. The levels of serum TGFβ1 were detected by ELISA. Results Compared with normal control group, CBI mRNA and FAK mRNA levels in every model group were significantly increased ( P ＜ O.05 ). With the modeling time prolonged, CB1 and FAK mRNA levels gradually increased (P ＜0.05 ). CB1 mRNA level in liver tissue not only had correlation with the degree of liver fibrosis ( r = O. 747, P ＜ 0.01 ), but also with FAK mRNA level in liver tissue ( r = 0.907, P ＜ O. 01 ). With the modeling time prolonged, serum TGFβ1 level gradually increased (P ＜ O. 05 ). CBI mRNA level in liver tissue had positive correlation with serum TGFβ1 level ( r = O. 542, P ＜ 0. O1 ). Conclusion CB1 may promote the activation of FAK in rats with fibrosis, and induce HSC proliferation and secrete plentiful TGFβ1 by PI3K signal transduction. CB1 may promote progression of liver fibrosis.关键词
受体,大麻酚,CBI/黏着斑蛋白酪氨酸激酶类/肝硬化Key words
receptor/ cannabinoid/ CB1/ focal adhesion protein -tyrosine kinases/ liver cirrhosis分类
医药卫生引用本文复制引用
杨莉,赵召霞,侯军良,刘玉珍,姜惠卿,戴二黑..大麻素受体1、FAK mRNA在小鼠肝纤维化形成过程中肝组织中的表达及相互关系[J].临床肝胆病杂志,2011,27(8):824-826,836,4.基金项目
中国肝炎防治基金会王宝恩肝纤维化研究基金(20080013) （20080013）
河北省卫生厅指令性课题(08190) （08190）
