临床肝胆病杂志2011，Vol.27Issue(8)：827-832,836,7.
胶囊渗透压泵控释rmIGFBPrP1对小鼠肝肾组织的影响及意义
The effect and significance of exogenous IGFBPrP1 on liver and kidney tissue in mice
摘要
Abstract
Objective To investigate the effect of exogenous recombinant mouse insulin - like growth factor binding protein related protein 1 ( rmIGFBPrP1 ) on both liver and kidney tissue in mouse, and explore the mechanism of rmIGFBPrP1 on fibrosis. Methods A total of 26 clean male wild -type C57BL/6 mice were randomly divided into normal control group, sham -operated group and model group. The rmIGFBPrP1 which was released by capsnle osmotic pump was used to make the model. HE staining and Sirius red staining were observed to investigate the pathological changes and deposition of collagen fibers in liver and kidney of mice. The expression and distribution of IGFBPrP1,Smod3, p - Smad2/3, Ⅲ collagen ( Collagen Ⅲ) of liver and kidney tissues were detected by immunohistochemistry. Hepatocyte apoptosis was detected by DUTP nick end labeling ( TUNEL). Results Comparing with the control group and sham - operated group, the content of collagen fiber and the expression of IGFBPrP1, Smad3, p - Smad2/3, Collagen Ⅲ increased ( P ＜0.01 ) in the liver of model group, While only the expression of IGFBPrP1 in the kidney of model group increased than that in the control group and sham - operated group ( P ＜0.01 ), there is no difference of collagen fibers content and other indicators expression in these three groups( P ＞0. 05 ). TUNEL showed the increase in ratio of apoptotic liver cells in model group than that in normal group and sham -operated group ( P ＜0.01 ). Conclusion Exogenous IGFBPrP1 can promote hepatocyte apoptosis and liver fibrosis, while have little effect on the mice kidney. IGFBPrP1 may selectively induce organs and organization fibrosis in mice.关键词
胰岛素样生长因子结合蛋白相关蛋白1/肝硬化/细胞凋亡Key words
insulin - like growth facter binding protein 1/ liver cirrhosis/ apoptosis分类
医药卫生引用本文复制引用
冯建文,刘立新,张海燕,张骞骞..胶囊渗透压泵控释rmIGFBPrP1对小鼠肝肾组织的影响及意义[J].临床肝胆病杂志,2011,27(8):827-832,836,7.基金项目
国家自然科学基金(30871146) （30871146）
山西省回国留学人员科研资助项目(2009-44) （2009-44）
