中国动脉硬化杂志2011，Vol.19Issue(7)：552-556,5.
tBHQ通过Nrf2通路抗脂多糖诱导人脐静脉内皮细胞损伤中的作用及机制
Effects of tBHQ on Lipoppolysacchride-Induced Impairment of Human Umbilial Vein Endothelial Cell by Nuclear Factor-Erythroid 2 p45-Related Factor 2
摘要
Abstract
Aim To investigate the role and mechanism of tert-butylhydroquinone (tBHQ) on lipoppolysacchride (LPS) -induced impairment of human umbilial vein endothelial cell (HUVEC). Methods Before treated with LPS,HUVEC were cultured with tBHQ in vitro. MTT analysis measured the effects of LPS and tBHQ on HUVEC; Western blot analysis measured the effects of tBHQ on the levels of Nrf2 in HUVEC; DCFH-DA measured the effects of tBHQ on the levels of ROS; Western blot and ELISA analysis measured the effects of tBHQ on the levels of TNF-α, IL-1 β and IL-6.Results Compared with control group, viabilities of HUVEC were decreased significantly by LPS (P ＜ 0. 05 ). However, the effects of LPS could be attenuated notably by tBHQ (P ＜ 0. 05 ). Pretreatment with tBHQ could increase the expression of nuclear Nrf2 protein significantly ( P ＜ 0.05 ), and reduce the levels of ROS, TNF-α, IL-1 β and IL-6 ( P ＜0. 05). Conclusions To prevent LPS-induced impairment of HUVEC, tBHQ can decrease the levels of ROS inflammatory factors induced by LPS via inducing translocation of Nrf2.关键词
叔丁基对苯二酚/转录因子NF-E2相关因子2/脂多糖/人脐静脉内皮细胞Key words
Tert-Butylhydroquinone/ Nuclear Factor-Erythroid 2 p45-Related Factor 2/ Lipoppolysacchride/ Human Umbilial Vein Endothelial Cell分类
医药卫生引用本文复制引用
曾慰,肖新华,胡名松,邓宏军,胡军,郑达扬,高文奎..tBHQ通过Nrf2通路抗脂多糖诱导人脐静脉内皮细胞损伤中的作用及机制[J].中国动脉硬化杂志,2011,19(7):552-556,5.基金项目
国家自然科学基金(81070667) （81070667）
