中国动脉硬化杂志2011，Vol.19Issue(7)：578-582,5.
血凝素样氧化型低密度脂蛋白受体1基因沉默抑制人脐静脉内皮细胞表达分形趋化因子和单核细胞趋化蛋白1
Lectin-Like Oxidized-LDL Receptor-1 Gene Silence Suppressed Expression of Fractalkine and Monocyte Chemoattractant Protein-1 Induced By Oxidized Low-Density Lipoprotein in Human Umbilical Vein Endothelial Cells
张社兵 1姜德谦 2欧柏青 2刘虹 2张小炼 2徐新 2马绍椿 1唐良秋1
作者信息
- 1. 广东省粤北人民医院心内科,广东省韶关市512026
- 2. 中南大学湘雅二医院心内科,湖南省长沙市410011
- 折叠
摘要
Abstract
Aim To observe effect of lectin-like oxidized low density lipoprotein(ox-LDL) receptor-1 ( LOX-1 )gene silence on the expressions of fractalkine and monocyte chemoattractant protein-1 ( MCP-1 ) induced by ox-LDL in human umbilical vein endothelial cells (HUVEC). Methods HUVEC were cultured and treated with ox-LDL of three different concentrations (25, 50, 100 mg/L respectively) for 24 hours; pGenesil-1 LOX-1 shRNA were transfected into HUVEC and then cells were treated with ox-LDL (50 mg/L). mRNA and protein expressions of LOX-1, fractalkine and MCP-1 were determined by semi-quantitative reverse-transcription polymerase chain reaction ( RT-PCR), Western Blot analysis and enzyme linked immunosorbent assay. Results Ox-LDL could induce mRNA and protein expressions of LOX-1, fractalkine and MCP-1 in a dose-dependent manner(P ＜ 0. 01 ). Expressions of fractalkine and MCP-1 induced by ox-LDL (50 mg/L) were significantly suppressed by transfection with LOX-1 specific shRNA(P ＜0. 01 ). Conclusions Ox-LDL can induce expressions of fractalkine and MCP-1 in a dose-dependent manner in HUVEC, and this process can be suppressed by LOX-1 gene silence.关键词
人脐静脉内皮细胞/RNA干扰/氧化型低密度脂蛋白/血凝素样氧化型低密度脂蛋白受体1/分形趋化因子/单核细胞趋化蛋白1Key words
Human Umbilical Vein Endothelial Cells/ RNA Interference/ Oxidized Low Density Lipoprotein/Lectin-Like Oxidized Low Density Lipoprotein Receptor-1/ Fractalkine/ Monocyte Chemoattractant Protein-1分类
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张社兵,姜德谦,欧柏青,刘虹,张小炼,徐新,马绍椿,唐良秋..血凝素样氧化型低密度脂蛋白受体1基因沉默抑制人脐静脉内皮细胞表达分形趋化因子和单核细胞趋化蛋白1[J].中国动脉硬化杂志,2011,19(7):578-582,5.
