中国动脉硬化杂志2011，Vol.19Issue(9)：716-720,5.
骨髓间充质干细胞移植对损伤血管的内皮修复作用
Effect of Bone Marrow Mesenchymal Stem Cells Transplantation on Repairment of Injured Vascular Endothelial
摘要
Abstract
Aim To investigate the effect and possible mechanism of bone marrow mesenchymal stem cells ( BMSC) transplation on reparing injured vessels endothelium after carotid atherosclerosis stenosis angioplasty in rabbits.Methods Carotid atherosclemsis stenosis model of 48 rabbits had been successfully built up and randomly divided into BMSC transplantation group ( n = 24) and control group ( n = 24). BMSC were obtained by density gradient centriffugation and adherent cultured. Surface markers of BMSC were detected by flow cytometry, and BMSC pre-labled by DAPI. Balloon injured carotid artery of rabbits, meanwhile, BMSC (107/kg) were infused into injured artery of BMSC transplantation group rabbits by external carotid artery, and control group infused the same amount of PBS solution. The peripheral blood was collected and the vascular endothelial growth factor (VEGF) levels was detected by enzyme linked immunosorbent assay (ELISA) at preoperative and 3, 7, 14, 28 days of BMSC transplantation. 7 days after BMSC transplantation, DAPI labeled BMSC were detected by immunofluorescence microscopy. 14 days after BMSC transplantation, the immunohistochemical staining was used to analyse platelet-endothelial cell adhesion molecule( CD31 )expression in the injured vessels.28 days after BMSC transplantation, the neointimal area, the ratio of the intima/media area and vascular restenosis were analysed in the vascular tissue by hematoxylin and eosin staining. Results The expression of VEGF in BMSC transplantation group were elevated significantly compared with control group at 3, 7, 14, 28 days after BMSC transplantation.7 days after BMSC transplantation, DAPI labeled BMSC were detected in injured vessels intima. 14 days after BMSC transplantation, CD31 continues to express in intima of BMSC transplantation group, while the control group did not express CD31. The neointimal area(0. 092 ±0. 009 vs0. 189 ±0.007, P＜0. 01), the ratio of the intima/media area (0.698 ± 1.570 vs 1.630±0. 122, P＜0. 01)and the luminal stenosis ratio(41.70％ ±3.70％ vs 61.28％ ± 1.57％, P＜0.01)were significantly decreased in BMSC transplantation group compared with control group at 28 days. Conclusion BMSC transplantation can promote repairing of endothelial after atherosclerotic stenosis carotid artery by balloon injury and reduce the restenosis of injured vessels.关键词
骨髓间充质干细胞/再狭窄/内皮修复Key words
Bone Marrow Mesenchymal Stem Cells/ Restenosis/ Repairment of Endothelial分类
医药卫生引用本文复制引用
刘志江,石蓓,许官学,沈长银,王正龙,龙仙萍,陈攀科..骨髓间充质干细胞移植对损伤血管的内皮修复作用[J].中国动脉硬化杂志,2011,19(9):716-720,5.基金项目
国家自然科学基金资助项目(NSFC30860100) （NSFC30860100）
