南方医科大学学报2011，Vol.31Issue(7)：1268-1272,5.
血小板衍生生长因子-BB在增生肥厚黄韧带中的表达及意义
Over-expression of platelet-derived growth factor-BB in degenerative hypertrophied ligamentum flavum
张宇 1许学猛 1吴少鹏 1夏雄智 1李参天 1邓崇礼 1陈建庭2
作者信息
- 1. 广东省第二中医院脊柱骨科,广东,广州,510095
- 2. 南方医科大学南方医院脊柱骨病外科,广东,广州,510515
- 折叠
摘要
Abstract
Objective To investigate the role of platelet-derived growth factor-BB (PDGF-BB) in the degeneration of the hypertrophied ligamentum flavum (LF) in the lumbar spine. Methods Surgical specimens of degenerative hypertrophied LF were obtained from 11 patients with lumbar spinal stenosis (LSS, mean age 57.8 years), with those from 10 age-matched patients with lumbar disc hemiation (LDH, mean age 53.5 years) as the normal controls. The thickness of the LF was measured preoperatively by axial Tl-weighted magnetic resonance imaging (MRI) at the facet joint level. Immunohistochemistry was employed to determine the expression of PDGF (3 and PDGF-BB in the LF. The mRNA and protein expressions of PDGF-BB in the LF were detected using real-time PCR and Western blotting, respectively. Results The thickness of the LF was 5.30±1.12 mm in the degenerative group and 2.80±1.53 mm in the control group, showing a significant difference between the two groups (P0.001). PDGF-β and PDGF-BB were positive in the fibroblasts in hypertrophied LF. The mRNA and protein expressions of PDGF-BB were significantly higher in the degenerative group than in the control group (P=0.013 and 0.023, respectively). Conclusion The high expression of PDGF-BB in the hypertrophied LF suggests its important role in the development of hypertrophy of LF in lumbar spinal canal stenosis.关键词
血小板衍生生长因子-BB/黄韧带增生肥厚/腰椎管狭窄症Key words
platelet-derived growth factor-BB/hypertrophied ligamentum flavum/lumbar spinal stenosis分类
医药卫生引用本文复制引用
张宇,许学猛,吴少鹏,夏雄智,李参天,邓崇礼,陈建庭..血小板衍生生长因子-BB在增生肥厚黄韧带中的表达及意义[J].南方医科大学学报,2011,31(7):1268-1272,5.
