南方医科大学学报2011，Vol.31Issue(7)：1179-1182,4.
补体应答基因-32过表达对SW480细胞骨架的影响
Overexpression of response gene to complement-32 promotes cytoskeleton reorganization in SW480 cell line
摘要
Abstract
Objective To construct the recombinant plasmid pcDNA3.0-RGC32 and evaluate the effect of the response gene to complement-32 (RGC32) on cell cytoskeleton in vitro. Methods The full-length cDNA of RGC32 was obtained by RT-PCR and inserted into the eukaryotic expression vector pcDNA3.0 to generate the recombinant plasmid pcDNA3.0-RGC32. After transfection of the recombinant plasmid into SW480 cells, the expression of RGC32 in the cells was detected by Western blotting. The cytoskeleton of SW480 cells was visualized before and after the transfection, and the changes in the cell migration ability was assessed by wound-healing assay. Results The recombinant plasmid pcDNA3.0-RGC32 was successfully constructed. The expression of RGC32 was significantly increased in SVV480 cells after transfection with pcDNA3.0-RGC32. Before the transfection, the microfilaments of SW480 cells were few and short without obvious polarity, but after the transfection, the microfilaments were increased and elongated with also an obvious polarity, and the invasive structures of lamellae and lamellipodia occurred. The migration ability of the cells was enhanced after transfection with pcDNA3.0-RGC32. Conclusion Overexpression of RGC32 can cause the reorganization of cytoskeleton and promotes the cell migration, which can be an important mechanism of RGC32 in promoting cancer metastasis.关键词
补体应答基因-32/细胞骨架/转移/结直肠癌/迁移能力Key words
RGC32/cytoskeleton/metastasis/colon cancer/cell migration分类
医药卫生引用本文复制引用
田杰,徐川,杨敏慧,李祖国..补体应答基因-32过表达对SW480细胞骨架的影响[J].南方医科大学学报,2011,31(7):1179-1182,4.基金项目
国家自然科学基金(30770976) （30770976）
