南方医科大学学报2011，Vol.31Issue(7)：1224-1227,4.
妊娠期糖尿病合并妊娠期高血压疾病血清差异蛋白的鉴定
Identification of protein markers for gestational diabetes mellitus complicated by pregnancy-induced hypertensive syndrome
摘要
Abstract
Objective To identify the serum protein markers for the gestational diabetes mellitus (GDM) complicated by pregnancy-induced hypertensive (PIH) syndrome to provide a molecular biological basis for the screening, prevention and therapy of the related diseases. Methods Serum samples were collected from the patients with GDM, PIH syndrome, and GDM complicated by PIH syndrome. IgG and albumins were removed from the samples before SDS-PAGE. The protein bands showing significant differences among the 3 samples were collected, digested and identified with mass spectrometry, and the function of the identified proteins was analyzed. Results Three SDS-PAGE were performed in parallel to confirm the differentially expressed proteins. Mass spectrometry indicated that the proteins showing obvious differences among the 3 samples were haptoglobin, protein SMG8 and apoptosis-inducing factor-1. Conclusions The protein markers identified in GDM complicated by PIH syndrome may be integrated into the proteomic database of gestational metabolic diseases. Identification of the associated protein markers may provide significant experimental data for the prevention, diagnosis and therapy of the related diseases.关键词
妊娠期糖尿病合并妊娠期高血压疾病/妊娠期高血压疾病/妊娠期糖尿病/差异蛋白/质谱Key words
gestational diabetes mellitus/complicated pregnancy induced hypertensive syndrome/pregnancy induced hypertensive/gestational diabetes mellitus/serum protein markers/mass spectrometry分类
医药卫生引用本文复制引用
王硕石,胡水旺,钟梅..妊娠期糖尿病合并妊娠期高血压疾病血清差异蛋白的鉴定[J].南方医科大学学报,2011,31(7):1224-1227,4.基金项目
广东省科技计划资助项目(20078030502015) （20078030502015）
