中国临床医学影像杂志2011，Vol.22Issue(8)：552-556,5.
18F-FDG与人卵巢癌细胞株SKOV3结合实验方法学研究
Studies on the methodology of 18F-FDG cell binding of ovarian cancer SKOV3
刘文平 1吴翼伟 2章斌 2邓胜明 2崔凤 1徐坤元1
作者信息
- 1. 杭州市中医院放射科,浙江,杭州,310007
- 2. 苏州大学附属第一医院核医学科,江苏,苏州,215006
- 折叠
摘要
Abstract
Objective: To establish a useful and stable methodology of 18F-FDG cell binding of ovarian cancer SKOV3. Methods: Several binding conditions were tested. ①The cell number was from 5×104 to 5×106 cells/bottle. ②The reaction time was from 20 to 120min. ③The radioactivity of 18F-FDG was from 1.85 to 29.6KBq. ④The glucose concentration was from 0 to 11.1mmol/L. The CPM (B) in cells and CPM (F) in supernatant were measured by the γ detector. The binding efficiency of ovarian cancer cell line SKOV3 with 18F-FDG was calculated. Results: ①There was linear regression relationship between 18FFDG cell binding efficiency of human ovarian cancer SKOV3(y) and the cell number(x1), reaction time(x2), glucose concentration (x3). Regression equation: y=(7.395+5.18E-0.06x1+0.094x2-2.040x3)×100％, F=106.9, P＜0.05. The P values of partial regression coefficient were less than 0.05. R2c=0.728, P＜0.05. The Pearson correlation coefficient of cell number, reaction time, glucose concentration with 18F-FDG cell binding efficiency was 0.581、0.179、-0.599 respectively, P＜0.05. ②The basic conditions of 18F-FDG cell binding of ovarian cancer SKOV3: the cell number was 1 ×106 cells/bottle, the radioactivity of 18F-FDG was 3.7KBq, the glucose concentration was 0mmol/L, the reaction time was 100min. The binding efficiency was (25.45±1.66)％. Conclusions: The methodology of 18F-FDG cell binding of ovarian cancer SKOV3 was established successfully. It contributed to evaluate early the effect of radiotherapy and chemotherapy on inhibiting ovarian cancer cell line SKOV3.关键词
卵巢肿瘤/氟脱氧葡萄糖F18Key words
Ovarian neoplasms/ Fluorodeoxyglucose F18分类
医药卫生引用本文复制引用
刘文平,吴翼伟,章斌,邓胜明,崔凤,徐坤元..18F-FDG与人卵巢癌细胞株SKOV3结合实验方法学研究[J].中国临床医学影像杂志,2011,22(8):552-556,5.
